engleski

Optimization of semi solid bioprocess performance for improvement of bioethanol production

Biotechnology of biofuels production from lignocellulose material comes in the focus of interest in few last decades. Since than many investigations were conducted to promote efficient and sustainable technology for biofuels production as well as economically feasible bioprocesses. Mathematical modelling and the optimisation of operations have the potential for improvement of biofuels production. In this investigation experimental design and multi parameter optimisation procedures were used in the preparation of multy-enzyme solution from Trametes versicola, Neurospora crasa and Screlotium rolfsii. It was found that that the combination of pectinolytic, hemicellulolylic and cellulolytic activities works synergistically on the complex lignocelluloses composition and enable achieving a high degree of enzymatic lignocellulose hydrolysation with a low enzyme load. Selected enzyme solution was tested in the hydrolysation experiments of sugar beet pulp (SBP) using two bioreactor types: stirred tank bioreactors (STR) and horizontal rotating tubular bioreactor (HRTB). Semi solid state fermentation (sSSF) in HRTB had higher bioprocess performance compare to the STR due to the HRTB construction characteristics and advantages of solid state fermentation. Partial differential equation (PDE) model of bioethanol production in HRTB was developed. This model combines mixing, diffusion and kinetic model ant it gives an insight how reaction and transport phenomena together with operating variables could affected bioprocess performance.

Semi solid state fermentation; bioethanol; bioreactor; mathematical modelling

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