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Pregled bibliografske jedinice broj: 77301

Macromolecular Architecture in Eukaryotic Cells Visualized by Cryoelectron Microscopy


Medalia, O.; Weber, Igor; Frangakis, A.S.; Nicastro, D.; Gerisch, G.; Baumeister, W.
Macromolecular Architecture in Eukaryotic Cells Visualized by Cryoelectron Microscopy // Science, 298 (2002), 1209-1213 (međunarodna recenzija, članak, znanstveni)


Naslov
Macromolecular Architecture in Eukaryotic Cells Visualized by Cryoelectron Microscopy

Autori
Medalia, O. ; Weber, Igor ; Frangakis, A.S. ; Nicastro, D. ; Gerisch, G. ; Baumeister, W.

Izvornik
Science (0036-8075) 298 (2002); 1209-1213

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Sažetak
Electron tomography of vitrified cells is a noninvasive three-dimensional imaging technique that opens up new vistas for exploring the supramolecular organization of the cytoplasm. We applied this technique to Dictyostelium cells, focusing on the actin cytoskeleton. In actin networks reconstructed without prior removal of membranes or extraction of soluble proteins, the cross-linking of individual microfilaments, their branching angles, and membrane attachment sites can be analyzed. At a resolution of 5 to 6 nanometers, single macromolecules with distinct shapes, such as the 26S proteasome, can be identified in an unperturbed cellular environment.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekt / tema
0098073

Ustanove
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Igor Weber (autor)

Citiraj ovu publikaciju

Medalia, O.; Weber, Igor; Frangakis, A.S.; Nicastro, D.; Gerisch, G.; Baumeister, W.
Macromolecular Architecture in Eukaryotic Cells Visualized by Cryoelectron Microscopy // Science, 298 (2002), 1209-1213 (međunarodna recenzija, članak, znanstveni)
Medalia, O., Weber, I., Frangakis, A., Nicastro, D., Gerisch, G. & Baumeister, W. (2002) Macromolecular Architecture in Eukaryotic Cells Visualized by Cryoelectron Microscopy. Science, 298, 1209-1213.
@article{article, year = {2002}, pages = {1209-1213}, keywords = {}, journal = {Science}, volume = {298}, issn = {0036-8075}, title = {Macromolecular Architecture in Eukaryotic Cells Visualized by Cryoelectron Microscopy}, keyword = {} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • Biological Abstracts
  • Chemical Abstracts