Macromolecular Architecture in Eukaryotic Cells Visualized by Cryoelectron Microscopy (CROSBI ID 94185)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Medalia, Ohad ; Weber, Igor ; Frangakis, Achilleas S. ; Nicastro, Daniela ; Gerisch, nther ; Baumeister, Wolfgang
engleski
Macromolecular Architecture in Eukaryotic Cells Visualized by Cryoelectron Microscopy
Electron tomography of vitrified cells is a noninvasive three-dimensional imaging technique that opens up new vistas for exploring the supramolecular organization of the cytoplasm. We applied this technique to Dictyostelium cells, focusing on the actin cytoskeleton. In actin networks reconstructed without prior removal of membranes or extraction of soluble proteins, the cross-linking of individual microfilaments, their branching angles, and membrane attachment sites can be analyzed. At a resolution of 5 to 6 nanometers, single macromolecules with distinct shapes, such as the 26S proteasome, can be identified in an unperturbed cellular environment.
electron tomography ; Dictyostelium cells ; actin cytoskeleton ; eukaryotic cells
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