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DDPCR and RACE on developing lymphocytes reveal a new mouse gene

Genes differentially transcribed during development of murine thymocytes were studied. We used differential display of mRNA by polymerase chain reaction (DD-PCR) and rapid amplification of DNA ends (RACE) and identified a cDNA for U2snRNP-A' from a transcript abundant in precursor thymocytes, but rare in mature T cells. Precursor thymocytes were isolated by flow cytometry multiparametar analysis and sorting on the basis of CD4, CD8, CD3 and lineage antigen expression. The transcript was cloned and it is homologous to human cDNA for U2. We found this gene most abundantly transcribed on day 15 of gestation and in adult prothymocytes, spleen, testis and liver. We also analysed U2 snRNP-A' gene expression in different lymphocyte subpopulations from thymus, lymph nodes, spleen and bone marrow. Further characterisation of snRNP proteins in the mouse is warranted in an effort to establish animal models of autoimmunity relevant for studies of connective tissue diseases or systemic lupus erythematosus where patients harbour autoantibodies reactive to snRNP.

T lymphocytes; Differetntial expression; DD-PCR; RACE; U2 snRNP-A' gene

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