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Pregled bibliografske jedinice broj: 772650

Characterisation of Brucella suis isolates from Southeast Europe by multi-locus variable-number tandem repeat analysis


Duvnjak, Sanja; Račić, Ivana; Špičić, Silvio; Zdelar-Tuk, Maja; Reil, Irena; Cvetnić, Željko
Characterisation of Brucella suis isolates from Southeast Europe by multi-locus variable-number tandem repeat analysis // Veterinary microbiology, 180 (2015), 1-2; 146-150 doi:10.1016/j.vetmic.2015.08.013 (međunarodna recenzija, članak, znanstveni)


Naslov
Characterisation of Brucella suis isolates from Southeast Europe by multi-locus variable-number tandem repeat analysis

Autori
Duvnjak, Sanja ; Račić, Ivana ; Špičić, Silvio ; Zdelar-Tuk, Maja ; Reil, Irena ; Cvetnić, Željko

Izvornik
Veterinary microbiology (0378-1135) 180 (2015), 1-2; 146-150

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Brucella suis; MLVA; genotipizacija; Hrvatska; JI Europa; zarazna bolest
(Brucella suis; MLVA; genotyping; Croatia; SE Europe; infectious disease)

Sažetak
Porcine brucellosis is a common bacterial zoonosis which can cause significant financial losses. Its diverse and often complicated factors have hampered efforts to control disease spread. The aim of the study was to assess the epidemiological situation of porcine brucellosis primarily in Croatia and its relationship to genotypes present in other, mostly European countries. One hundred and seven Brucella suis strains isolated from swine, hares, cattle, humans, wild hares, a wild boar and a mare originating mainly from Croatia (112), but also a few from Slovenia, Bosnia and Herzegovina, Serbia and Macedonia (15) were tested using classical microbiological testing, Bruce-ladder, RFLP, Multiplex-suis and genotyped using multi-locus variable-number tandem repeat analysis (MLVA). We determined 43 Brucella suis genotypes. Strains were grouped according to phylogenetic and geographic relationships, revealing both regional specificity and uniqueness and suggesting possible sources and modes of spread among animals. Our study also confirmed problems with Bruce19 locus that may hinder comparisons of new types with those in the international database. Forty-one novel genotypes were identified and deposited into the international database. Our study supports the idea of wild animals as a source of disease in domestic animals and also gives evidence to hypothesis of cross-border animal trafficking between former Yugoslavian countries. It also highlights the need to expand such research across more of southeast Europe, especially to countries with poorer social and economical situation in order to prevent a realistic outbreak and for better understanding of the biology of this pathogen.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Veterinarska medicina



POVEZANOST RADA


Projekt / tema
048-0481153-1150 - Molekularna epizootiologija važnih bakterijskih zoonoza (Željko Cvetnić, )

Ustanove
Hrvatski veterinarski institut, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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