engleski

Helios gene expression in human lymphoproliferative disorders

The Ikaros transcription factor family is a family of related genes that takes a critical part in lymphocyte development. Transcription factors regulate expression of genes whose products influence lymphocyte development and therefore have a crucial role the lymphoid cell fate.Recently, a new member was discovered - Helios transcription factor. Given the predicted significance of these transcription factors in lymphocyte maturation, the aim of this study was to investigate Helios expression in human lymphocytes and in human lymphoproliferative disorders. We amplified human RNA using primers complementary to the murine Helios mRNA and primers complementary to the human Helios mRNA. RT-“nested” PCR products from the reactions with murine primers were the same size as the ones derived from mice (Helios A, 712 bp i Helios B 790 bp) what indicates great homology between human and murine Helios. Using murine primers, in human peripheral blood lymphocytes we managed to detect only the Helios A isoform and in lymph nodes and T lymphocyte derived cell lines both known isoforms. However, in lymphocytes derived from lymphoma patients there were only RT-PCR products of a very small size (150 bp). By means of RT-PCR with human primers we amplified two isoforms of Helios (202 bp and 100 bp) in human peripheral blood lymphocytes and in lymph nodes, while we detected different isoforms in different T lymphocyte derived cell lines. As well as in reactions with murine primers in lymphocytes derived from lymphoma patients there were also RT-PCR products of a very small size (100 bp). It is possible that these small products represent yet unknown isoforms of Helios or they reflect a mutation or deletion in the Helios gene of these patients.

Helios; lymphocyte development; transcription factors

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