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Pregled bibliografske jedinice broj: 772077

Improved separation and analysis of glycolipids by Iatroscan thin–layer chromatography–flame ionization detection


Gašparović, Blaženka; Kazazić, Snježana P.; Cvitešić, Ana; Penezić, Abra; Frka, Sanja
Improved separation and analysis of glycolipids by Iatroscan thin–layer chromatography–flame ionization detection // Journal of chromatography. A, 1409 (2015), 259-267 doi:10.1016/j.chroma.2015.07.047 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 772077 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Improved separation and analysis of glycolipids by Iatroscan thin–layer chromatography–flame ionization detection

Autori
Gašparović, Blaženka ; Kazazić, Snježana P. ; Cvitešić, Ana ; Penezić, Abra ; Frka, Sanja

Izvornik
Journal of chromatography. A (0021-9673) 1409 (2015); 259-267

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
sulfoquinovosyldiacylglycerol (SQDG) ; monogalactosyldiacylglycerol (MGDG) ; digalactosyldiacylglycerol (DGDG) ; Iatroscan thin–layer chromatography

Sažetak
We demonstrate improved power of Iatroscan thin layer chromatography/flame ionization detection (TLC-FID) technique for analysis of complex marine lipid mixture by developing protocol for the separation and analysis of glycolipids including sulfoquinovosyldiacylglycerols (SQDG), monogalactosyldiacylglycerols (MGDG) and digalactosyldiacylglycerols (DGDG). We have modified the common protocol used so far for the analysis of lipid classes by replacing the elution step which uses pure acetone for the elution of acetone mobile polar lipids, with the elution step containing chloroform–acetone (72:28, v:v) for separation of MGDG and DGDG. To separate SQDG from the complex lipid matrix we introduced solvent mixture acetone–chloroform–methanol–formic acid (33:33:33:0.6, v:v:v:v). Quantification of glycolipid classes was performed after calibration with glycolipid standards for the masses between 0.2 and 4 µg. With this new protocol we have successfully separated three glycolipids from the complex particulate lipid mixture of the seawater samples. Such an approach extends the power of existing protocol for the analysis of lipids which altogether ensure detection and quantification of 18 lipid classes what was demonstrated on seawater samples. This enables to gain a very broad system overview of the particularly complex environments as are seas, oceans and freshwaters.

Izvorni jezik
Engleski

Znanstvena područja
Geologija



POVEZANOST RADA


Projekti:
HRZZ-IP-2013-11-8607 - Utjecaj okolišnog stresa na pojavnost i međudjelovanje biološki važnih organskih molekula i mikronutrijenata u morskom ekosustavu (AMBIOMERES) (Gašparović, Blaženka, HRZZ - 2013-11) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Citiraj ovu publikaciju

Gašparović, Blaženka; Kazazić, Snježana P.; Cvitešić, Ana; Penezić, Abra; Frka, Sanja
Improved separation and analysis of glycolipids by Iatroscan thin–layer chromatography–flame ionization detection // Journal of chromatography. A, 1409 (2015), 259-267 doi:10.1016/j.chroma.2015.07.047 (međunarodna recenzija, članak, znanstveni)
Gašparović, B., Kazazić, S., Cvitešić, A., Penezić, A. & Frka, S. (2015) Improved separation and analysis of glycolipids by Iatroscan thin–layer chromatography–flame ionization detection. Journal of chromatography. A, 1409, 259-267 doi:10.1016/j.chroma.2015.07.047.
@article{article, year = {2015}, pages = {259-267}, DOI = {10.1016/j.chroma.2015.07.047}, keywords = {sulfoquinovosyldiacylglycerol (SQDG), monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), Iatroscan thin–layer chromatography}, journal = {Journal of chromatography. A}, doi = {10.1016/j.chroma.2015.07.047}, volume = {1409}, issn = {0021-9673}, title = {Improved separation and analysis of glycolipids by Iatroscan thin–layer chromatography–flame ionization detection}, keyword = {sulfoquinovosyldiacylglycerol (SQDG), monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), Iatroscan thin–layer chromatography} }
@article{article, year = {2015}, pages = {259-267}, DOI = {10.1016/j.chroma.2015.07.047}, keywords = {sulfoquinovosyldiacylglycerol (SQDG), monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), Iatroscan thin–layer chromatography}, journal = {Journal of chromatography. A}, doi = {10.1016/j.chroma.2015.07.047}, volume = {1409}, issn = {0021-9673}, title = {Improved separation and analysis of glycolipids by Iatroscan thin–layer chromatography–flame ionization detection}, keyword = {sulfoquinovosyldiacylglycerol (SQDG), monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), Iatroscan thin–layer chromatography} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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