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VALIDATION OF TWO PRESUMPTIVE TESTS FOR BLOOD (CROSBI ID 626233)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Makar, Adela ; Merkaš, Siniša ; Uvodić, Petra ; Kozar-Filić, Jelena ; Ortynski, Nataša ; Crnjac, Josip ; Papić, Sunčica ; Popović, Maja ; Boričević, Ivana ; Abramović, Marko Krešimir et al. VALIDATION OF TWO PRESUMPTIVE TESTS FOR BLOOD // 9th ISABS, Book of abstracts. 2015. str. 115-115

Podaci o odgovornosti

Makar, Adela ; Merkaš, Siniša ; Uvodić, Petra ; Kozar-Filić, Jelena ; Ortynski, Nataša ; Crnjac, Josip ; Papić, Sunčica ; Popović, Maja ; Boričević, Ivana ; Abramović, Marko Krešimir ; Mršić, Gordan

engleski

VALIDATION OF TWO PRESUMPTIVE TESTS FOR BLOOD

Combur3 Test® E (Roche Diagnostics GmbH, Germany) and Hemastix® (Siemens Healthcare Diagnostics Inc, USA) are used as presumptive tests for blood. In this study, sensitivity and specificity of two tests was examined. Dilutions of blood (from 1:2 to 1:1 000 000) was tested with both tests. Blood on six different substrates was tested directly and after exposure to environemental conditions for 7 days. Additionally, the effect of detergent, bleach and stain removal on white cotton textile with blood was tested. Many common household and chemical substances, as well as substrates that are frequently seen in routine forensic casework, were tested for false positive reaction. Blood samples were tested for DNA to determine whether the presumptive tests, environemental conditions and chemicals damaged or destroyed DNA. Short tandem repeats (STRs) analysis was performed using AmpFlSTR NGM kit (Applied Biosystems, USA). Both tests had a sensitivity of 1:25 000. No effect of different substrates and chemicals on testing results was detected. DNA was recovered and amplified from blood diluted 1:10 000 and from all other blood samples that were tested. Many common household and natural substances, chemical substances and substances which can be mistaken for blood that were tested showed false positive results with both tests.

Forensics ; Genetics ; Validation ; Combur ; Hemastix

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o prilogu

115-115.

2015.

nije evidentirano

objavljeno

Podaci o matičnoj publikaciji

9th ISABS, Book of abstracts

Podaci o skupu

9th ISABS Conference on Forensic, Anthropologic Genetics and Mayo Clinic Lectures in Individualized Medicine

poster

01.01.2015-01.01.2015

Bol, Hrvatska

Povezanost rada

Biologija, Temeljne medicinske znanosti