engleski

Animal hair as a source of human DNA

Animal traces are becoming more frequent object of investigation in different forensic and legal cases. The most common animals that can be found in these cases are pets or domesticated animals, such as dogs, cats, parrots, sheep’s etc. In this study, hair samples from 4 different breeds of dogs and sheep’s were obtained. Human DNA samples from three different persons were deposited by rubbing the animal hair samples. For isolation of human DNA two different methods were used, Chelex®100 method and EZ1 Investigator Kit. Quantification of DNA was performed using Quantifiler® Human DNA Quantification Kit and Investigator Quantiplex kit, respectively. Amplification was performed using AmpFlSTR®NGMTM PCR Amplification Kit. Only male partial or full DNA profiles were obtained from animal hair samples. Results pointed out that Chelex®100 shouldbe a preferable method for isolation of human DNA when working with these kinds of forensic samples.

Forensics ; Genetics ; Touch DNA ; Chelex ; EZ1 Investigator

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