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Inherent variability in the kinetics of autocatalytic protein self-assembly


Szavits-Nossan, Juraj; Eden Kym; Morris, Ryan J.; MacPhee, Cait E.; Evans, Martin R.; Rosalind, Allen J.
Inherent variability in the kinetics of autocatalytic protein self-assembly // Physical Review Letters, 113 (2014), 098101-1 doi:10.1103/PhysRevLett.113.098101 (međunarodna recenzija, pismo, znanstveni)


Naslov
Inherent variability in the kinetics of autocatalytic protein self-assembly

Autori
Szavits-Nossan, Juraj ; Eden Kym ; Morris, Ryan J. ; MacPhee, Cait E. ; Evans, Martin R. ; Rosalind, Allen J.

Izvornik
Physical Review Letters (0031-9007) 113 (2014); 098101-1

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, pismo, znanstveni

Ključne riječi
Proteins; Self-Assembly; Kinetics; Fluctuations

Sažetak
In small volumes, the kinetics of filamentous protein self-assembly is expected to show significant variability, arising from intrinsic molecular noise. This is not accounted for in existing deterministic models. We introduce a simple stochastic model including nucleation and autocatalytic growth via elongation and fragmentation, which allows us to predict the effects of molecular noise on the kinetics of autocatalytic self-assembly. We derive an analytic expression for the lag-time distribution, which agrees well with experimental results for the fibrillation of bovine insulin. Our expression decomposes the lag-time variability into contributions from primary nucleation and autocatalytic growth and reveals how each of these scales with the key kinetic parameters. Our analysis shows that significant lag-time variability can arise from both primary nucleation and from autocatalytic growth and should provide a way to extract mechanistic information on early-stage aggregation from small-volume experiments.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Biologija



POVEZANOST RADA


Ustanove
Institut za fiziku, Zagreb

Autor s matičnim brojem:
Juraj Szavits Nossan, (285591)

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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