engleski

Genetic diversity and symbiotic efficiency of indigenous common bean rhizobia isolated from agricultural soils in the central Croatia

An important feature of sustainable agriculture is the effective management of nitrogen in the environment. Consequently, the natural process of symbiotic nitrogen fixation has a unique role in sustainable common bean production. The application of rhizobial inoculants enables the considerable reduction of mineral nitrogen what is of great ecological and economic importance. Nitrogen fixing capacity in agricultural systems depends on rhizobial strain, host plant and environmental conditions. It is well known that rhizobial strains strongly differ in their properties like symbiotic efficiency, competitiveness with other rhizobia in soil, compatibility with host plant, and adaptive ability to stress conditions in soil. Therefore, the selection of high quality rhizobial strains is one of the main presumptions for successfull inoculation. Furthermore, in order to maximize the contribution of biologically fixed nitrogen in soybean production, it is also very important to select the most effective symbiotic associations between common bean cultivar and rhizobial strain. However, indigenous rhizobial strains can be present in some soils. In specific agroecological conditions, these strains, usually show higher competitivity and adaptive ability in comparison with commercial strains while their symbiotic efficiency is usually unkonwn. Therefore, in order to improve the beneficial effect of common bean inoculation, it is important to determine the actual composition and the characteristics of rhizobial field population. The main aim of the present study was to identify and characterize the indigenous common bean rhizobia isolated from different field sites in central Croatia, to determine genetic diversity within rhizobial field population and to evaluate symbiotic properties of indigenous common bean rhizobia.

Nitrogen fixation ; Genetic diversity ; Common bean ; RAPD- PCR ; ERIC- PCR

nije evidentirano