engleski

Talens and CRISPR/Cas9: Generation of genome engineered mouse models using editable nucleases: Practical experience.

Targeting strategies based on programmable nucleases are a rapid and efficient system for site-specific knock-in and knock-out of gene of interest. These nucleases include zinc-finger nucleases (ZFN), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas RNA-guided nucleases. Therefore, the programmable nucleases are a method of choice for creation of mouse models where precision and speed is required. This presentation gives several practical examples of mouse models generated at the Czech Centre for Phenogenomics.

TALEN; CRISPR; mouse model

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