Comparison between the Comet assay and Fast Micromethod for measuring DNA damage in HeLa cells (CROSBI ID 94093)
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Podaci o odgovornosti
Bihari, Nevenka ; Batel, Renato ; Jakšić, Željko ; Mueller, W.E.G. ; Waldmann, Petra ; Zahn, R.K.
engleski
Comparison between the Comet assay and Fast Micromethod for measuring DNA damage in HeLa cells
The sensitivity and precision of the single cell gel electrophoresis (Comet) assay and Fast Micromethod for DNA damage determinations in human HeLa cell line were copared. Sensitivity and precision was the same and since the time required for multiple analysis by the Fast Micromethod is short (2 hours or less) its use in measuring DNA breakage in cells can be recommended for environmental genotoxicity monitoring.
Comet assay; Fast micromethod; DNA damage; HeLa cells; environment
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