engleski

Study of Influence of Laser Ablation Synthesized Silver Nanoparticles on E. Coli Bacteria

Laser ablation in liquids is a very convenient technique for production of nanoparticles free of chemical byproducts. Silver nanoparticles made in this way could show antimicrobial activity. For the purpose of this work we produced colloidal silver nanoparticles in water using a 1064 nm ns Nd:YAG laser at 100 mJ output energy and 5 Hz repetition rate. Size- distribution of nanoparticles was obtained from atomic force microscopy (AFM) images. We found the average diameter of silver nanoparticles to be around 10 nm with a relatively narrow size distribution. Obtained colloidal solution is stable on a scale of few months, which implies that nanoparticles are well dispersed and/or charged. We use this colloidal solution to investigate antimicrobial effects of silver nanoparticles on different strains of E. coli bacteria where the changes on bacterial membrane are monitored by AFM. Here we present our preliminary results.

Silver nanoparticles ; E. Coli ; Laser synthesis of nanoparticles

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