Capillary electrophoresis significantly improves clinical utility of hemoglobin A1c in gestational diabetes (CROSBI ID 625721)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Vučić Lovrenčić, Marijana ; Božičević, Sandra ; Krhač, Maja ; Radišić Biljak, Vanja ; Prašek, Manja
engleski
Capillary electrophoresis significantly improves clinical utility of hemoglobin A1c in gestational diabetes
BACKGROUND-AIM Clinical utility of hemoglobin A1c (Hb A1c) in diagnosis and monitoring of gestational diabetes (GDM) has been seriously compromised due to a lack of diagnostic accuracy originating from both biological variability and analytical limitations of contemporary methodology. Recently introduced capillary electrophoresis system for HbA1c analysis (Capillarys 2 Flex Piercing ®/ Minicap Flex Piercing®, Sebia, France) has been reported to have an excellent analytical performance and was found to be free from the common analytical interferences and hemoglobin variants, as well as fetal hemoglobin, which might be particularly interesting when measuring HbA1c in pregnancy complicated with diabetes. In this study we aimed to validate the clinical utility of HbA1c, as measured by the capillary electrophoresis, in diagnosis of GDM. METHODS 256 pregnant women (mean gestational age: 26±4, 7 weeks) were screened for GDM with a standard 75g oral glucose tolerance procedure followed by the venous plasma glucose measurement (hexokinase ; Beckman Coulter AU680, USA) at fasting, 1h and 2h after glucose load. Their glycaemic status was classified according to the WHO-2013 criteria. HbA1c was sampled at fasting and assayed with capillary electrophoresis [HbA1c- CAP (Minicap Flex Piercing®, Sebia, France)] and an automated immunoturbidimetric procedure [HbA1c-IT (TinaQuant-Integra 400Plus, Roche Diagnostics, USA)]. RESULTS GDM was diagnosed in 91 women (35, 5%), who did not differ regarding age and gestational age from women with normoglycaemia (NG ; N=165). HbA1c-CAP was significantly lower than HbA1c-IT (4, 7±0, 30%/28±3, 3 mmol/mol vs. 5, 2±0, 24%/33±2, 6 mmol/mol, P<0, 0001), and both systematic and proportional differences were found between the methods (y=1, 625+0, 750x ; intercept A/95%CI=1, 625/1, 233-2, 033 ; slope B/95%CI=0, 75/0, 667-0, 833 ; Passing Bablok). ROC- curve comparison showed a significantly better diagnostic accuracy of HbA1c-CAP vs. HbA1c-IT in discriminating between GDM and NG: [AUC/sensitivity(%)/specificity(%)/criterion(% /mmol/mol)=0, 727/70, 5/69/>4, 7/>28 vs. 0, 681/53, 7/74, 2/>5, 2/>33, respectively ; P=0, 0176]. CONCLUSIONS Our study indicates a significant improvement in clinical utility of hemoglobin A1c in GDM diagnosis with the use of capillary electrophoresis.
HbA1c; capillary electrophoresis; gestational diabetes
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o prilogu
S652-S652.
2015.
nije evidentirano
objavljeno
Podaci o matičnoj publikaciji
Clinical chemistry and laboratory medicine
Berlin: Walter de Gruyter
1437-4331
Podaci o skupu
EuroMedLab-2015
poster
21.06.2015-26.06.2015
Pariz, Francuska