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Analytical characterization and quantification of histidine dipeptides, carnosine and anserine by modeling of potentiometric titration data

In this paper, the relevant acid-base properties of the biologically important dipeptides carnosine and anserine were determined using potentiometric titration. The data obtained were used to calculate the dissociation constants of both carnosine and anserine. Both dipeptides in their fully protonated forms dissociate in three steps with corresponding dissociation constants of pKa1= 2.57, pKa2= 6.71 and pKa3= 9.57 for carnosine and pKa1= 2.51, pKa2= 6.97 and pKa3= 9.51 for anserine. The distribution diagram of the dissociated species as well as the buffer strength diagrams of carnosine and anserine provide useful information about their behaviors at different pH values. All computations were optimized via mathematical modeling with the Solver software.

dipeptide; carnosine; anserine; potentiometric titration; modeling; Solver optimization

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