engleski

Antiproliferative effect of non-aromatic oil fractions on squamous cell carcinoma VII: in vitro and preliminary in vivo results

Background and Purpose: Naphtalane, special petroleum oil cery rich in steranes, has been used for decades for treating patients with dermatological and locomotor diseases. Due to naphtalane`s promising clinical results in treating patients with psoriasis, and due to its structure which is very similar to corticosteroids and vitamin D, the aim of the study was to investigate the antiproliferative properties of three groups of preparations processed from oil from Križ near Ivanić Grad (Croatia). Aromatic compounds present in this oil were removed from the tested preparations, because they could be potentially carcinogenic. Materials and Methods: Squamous cell carcinoma (SCC VII) adherent cell cultures were exposed to three preparations derived from original oil. Single preparations and the combinations of all of them were used in the final concentrations of 10%, 1% and0.1%. Experiments were performed on murine fibroblast L929 cell cultures. Besides in vitro experiments, in vivo effect was studied on CH3 mice, which were injected with suspension of SCC VII cells. The tumor growth dynamics was recorded. Results: In vitro results showed marked dose-dependent cell proliferation inhibition of SCC VII cells, determined by 3H thymidine incorporation. The highest concentrations used, depending on investigated fraction, inhibited 3H thymidine incorporation from 65.5% to 98.3%, compared to the controls. The SCC VII cells were viable after exposition, so the effect is described as inhibitory rather than cytotoxic. However, the same preparations didn`t affect proliferation of L929 cells. In vitro results did not prevent tumor development, but inhibited its growth and delayed tumor nodule forming in the treated group for 14 days compared to controls. Conclusion: Non-aromatic compounds derived from natural oil have remarkable bioactive effects which deserve further attention in order to optimize their possible clinical applicability.

non-aromatic naphtalane oil; steranes; hopanes; antiproliferative effect; squamous cell carcinoma; cell cultures; C3H mice

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