Napredna pretraga

Pregled bibliografske jedinice broj: 767227

Synthetic vs natural scaffolds for human limbal stem cell cultivation


Tominac Trcin, Mirna; Dekaris, Iva; Mijović, Budimir; Bujić, Marina; Zdraveva, Emilija; Dolenec, Tamara; Pauk-Gulić, Maja; Primorac, Dragan; Crnjac, Josip; Špoljarić, Branimira et al.
Synthetic vs natural scaffolds for human limbal stem cell cultivation // Croatian medical journal, 56 (2015), 3; 246-256 doi:10.3325/cmj.2015.56.246 (međunarodna recenzija, članak, znanstveni)


Naslov
Synthetic vs natural scaffolds for human limbal stem cell cultivation

Autori
Tominac Trcin, Mirna ; Dekaris, Iva ; Mijović, Budimir ; Bujić, Marina ; Zdraveva, Emilija ; Dolenec, Tamara ; Pauk-Gulić, Maja ; Primorac, Dragan ; Crnjac, Josip ; Špoljarić, Branimira ; Mršić, Gordan ; Kuna, Krunoslav ; Špoljarić, Daniel ; Popović, Maja

Izvornik
Croatian medical journal (0353-9504) 56 (2015), 3; 246-256

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Scaffold ; Human ; Limbal stem cells

Sažetak
Aim was to investigate the impact of synthetic electrospun polyurethane and polycaprolactone nanoscaffolds, before and after hydrolytic surface modification, on viability and differentiation of cultured human eye epithelial cells, in comparison with natural scaffolds: fibrin and human amniotic membrane. Human placenta was taken at elective caesarean delivery. Fibrin scaffolds were prepared from commercial fibrin glue kits. Nanoscaffolds were fabricated by electrospinning. Limbal cells were isolated from surpluses of human cadaveric cornea and seeded on feeder 3T3 cells. The scaffolds used for viability testing and immunofluorescence analysis were: amniotic membrane, fibrin, polyurethane (PU) and polycaprolactone (PCL) nanoscaffolds, with or without prior NaOH treatment. SEM photographs of all tested scaffolds showed good colony spreading of seeded limbal cells. In regard to viability performance there was statistically significant difference between cells with highest viability cultured on tissue culture plastic and cells cultured on all other scaffolds. On the other hand, electrospun PU, PCL and electrospun PCL treated with NaOH had more than 80% of limbal cells positive for stem cell marker p63 compared to only 27%of p63 positive cells on fibrin. Natural scaffolds, fibrin and amniotic membrane, showed better cell viability compared to electrospun scaffolds. On contrary, high percentages of p63 positive cells on these scaffolds still makes them good candidates as efficient delivery systems for therapeutic purposes.

Izvorni jezik
Engleski

Znanstvena područja
Tekstilna tehnologija, Temeljne medicinske znanosti, Biotehnologija



POVEZANOST RADA


Ustanove
Veterinarski fakultet, Zagreb,
Šumarski fakultet, Zagreb,
Tekstilno-tehnološki fakultet, Zagreb,
KBC "Sestre Milosrdnice",
Sveučilište u Splitu

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati