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Two-step positioning of a cleavage furrow by cortexillin and myosin II (CROSBI ID 94047)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Weber, Igor ; Neujahr, R. ; Du, A. ; Köhler, J. ; Faix, J. ; Gerisch, G. Two-step positioning of a cleavage furrow by cortexillin and myosin II // Current biology, 10 (2000), 9; 501-506-x

Podaci o odgovornosti

Weber, Igor ; Neujahr, R. ; Du, A. ; Köhler, J. ; Faix, J. ; Gerisch, G.

engleski

Two-step positioning of a cleavage furrow by cortexillin and myosin II

Background: Myosin II, a conventional myosin, is dispensable for mitotic division in Dictyostelium if the cells are attached to a substrate, but is required when the cells are growing in suspension. Only a small fraction of myosin Il-null cells fail to divide when attached to a substrate. Cortexillins are actin-bundling proteins that translocate to the midzone of mitotic cells and are important for the formation of a cleavage furrow, even in attached cells. Here, we investigated how myosin II and cortexillin I cooperate to determine the position of a cleavage furrow. Results: Using a green fluorescent protein (GFP)-cortexillin I fusion protein as a marker for priming of a cleavage furrow, we found that positioning of a cleavage furrow occurred in two steps. In the first step, which was independent of myosin II and substrate, cortexillin I delineated a zone around the equatorial region of the cell. Myosin II then focused the cleavage furrow to the middle of this cortexillin I zone. If asymmetric cleavage in the absence of myosin II partitioned a cell into a binucleate and an anucleate portion, cell-surface ruffles were induced along the cleavage furrow, which led to movement of the anucleate portion along the connecting strand towards the binucleate one. Conclusions: In myosin II-null cells, cleavage furrow positioning occurs in two steps: priming of the furrow region and actual cleavage, which may proceed in the middle or at one border of the cortexillin ring. A control mechanism acting at late cytokinesis prevents cell division into an anucleate and a binucleate portion, causing a displaced furrow to regress if it becomes aberrantly located on top of polar microtubule asters.

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Podaci o izdanju

10 (9)

2000.

501-506-x

objavljeno

0960-9822

Povezanost rada

Biologija

Indeksiranost