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Pregled bibliografske jedinice broj: 76673

Dictyostelium DAip1, a homologue of the yeast actin-interacting protein 1, is involved in endocytosis, cytokinesis and motility


Konzok, A.; Weber, Igor; Simmeth, E.; Hacker, U.; Maniak, M.; Müller-Taubenberger, A.
Dictyostelium DAip1, a homologue of the yeast actin-interacting protein 1, is involved in endocytosis, cytokinesis and motility // Journal of Cell Biology, 146 (1999), 2; 453-464 (međunarodna recenzija, članak, znanstveni)


Naslov
Dictyostelium DAip1, a homologue of the yeast actin-interacting protein 1, is involved in endocytosis, cytokinesis and motility

Autori
Konzok, A. ; Weber, Igor ; Simmeth, E. ; Hacker, U. ; Maniak, M. ; Müller-Taubenberger, A.

Izvornik
Journal of Cell Biology (0021-9525) 146 (1999), 2; 453-464

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Sažetak
The 64-kD protein DAip1 from Dictyostelium contains nine WD40-repeats and is homologous to the actin-interacting protein 1, Aip1p, from Saccharomyces cerevisiae, and to related proteins from Caenorhabditis, Physarum, and higher eukaryotes. We show that DAip1 is localized to dynamic regions of the cell cortex that are enriched in filamentous actin: phagocytic cups, macropinosomes, lamellipodia, and other pseudopodia. In cells expressing green fluorescent protein (GFP)-tagged DAip1, the protein rapidly redistributes into newly formed cortical protrusions. Functions of DAip1 in vivo were assessed using null mutants generated by gene replacement, and by overexpressing DAip1. DAip1-null cells are impaired in growth and their rates of fluid-phase uptake, phagocytosis, and movement are reduced in comparison to wildtype rates. Cytokinesis is prolonged in DAip1-null cells and they tend to become multinucleate. On the basis of similar results obtained by DAip1 overexpression and effects of latrunculin-A treatment, we propose a function for DAip1 in the control of actin depolymerization in vivo, probably through interaction with cofilin. Our data suggest that DAip1 plays an important regulatory role in the rapid remodeling of the cortical actin meshwork.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekt / tema
00981005

Ustanove
Institut "Ruđer Bošković", Zagreb

Autor s matičnim brojem:
Igor Weber, (162634)

Citiraj ovu publikaciju

Konzok, A.; Weber, Igor; Simmeth, E.; Hacker, U.; Maniak, M.; Müller-Taubenberger, A.
Dictyostelium DAip1, a homologue of the yeast actin-interacting protein 1, is involved in endocytosis, cytokinesis and motility // Journal of Cell Biology, 146 (1999), 2; 453-464 (međunarodna recenzija, članak, znanstveni)
Konzok, A., Weber, I., Simmeth, E., Hacker, U., Maniak, M. & Müller-Taubenberger, A. (1999) Dictyostelium DAip1, a homologue of the yeast actin-interacting protein 1, is involved in endocytosis, cytokinesis and motility. Journal of Cell Biology, 146 (2), 453-464.
@article{article, year = {1999}, pages = {453-464}, keywords = {}, journal = {Journal of Cell Biology}, volume = {146}, number = {2}, issn = {0021-9525}, title = {Dictyostelium DAip1, a homologue of the yeast actin-interacting protein 1, is involved in endocytosis, cytokinesis and motility}, keyword = {} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • Biological Abstracts