engleski

Localization of trefoil factor family peptide 1 and 3 in epithelial tissues originating from all three primary germ layers of developing mouse embryo

Trefoil Factor Family (TFF) peptides are involved in the maintenance of epithelial integrity, and are important for epithelial restitution upon injury. Mature epithelial tissues originate from different primary germ layers in an embryo. Our aim was to investigate the presence and localization of TFF1 and TFF3 peptides in epithelial tissues of mouse embryo. Mouse embryos, day 14 to 18 were isolated and fixed in 4% paraformaldehyde. Paraffin blocks were made, cut into 6µm slides, and processed for manual immunohistochemical staining (LSAB method). Primary polyclonal, affinity purified rabbit anti-TFF1 and anti-TFF3 antibodies were used, and PBS as a negative control. TFF1 and TFF3 were present in different epithelial tissues, including stratified epithelia of the developing skin and vestibulum of oral cavity, gastrointestinal and respiratory mucosa, pancreas and kidney tubules with their occurence depending on the stage of embryonic development and tissue differentiation. Localization of TFF1 and TFF3 peptides in epithelial tissues of developing mouse embryo did not show germ layer-specific distribution. TFF1 and TFF3 promote migration of epithelial cells, participate in immune response, and affect apoptosis. Since epithelial tissues are characterized by active proliferation and TFF1 and TFF3 peptides are present in various epithelial tissues during embryonic development, they could play important roles in these tissues, and in some cases may even contribute to the differentiation of specific cells. Also, TFF1 and TFF3 may be considered markers of mucosal cells maturation.

Trefoil factor; embryonic development; mice

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