PCR-RFLP genotyping of two beta-fibrinogen gene polymorphisms (CROSBI ID 482891)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija
Podaci o odgovornosti
Begonja, Antonija ; Topić, Elizabeta ; Šimundić, Ana-Maria ; Štefanović, Mario
engleski
PCR-RFLP genotyping of two beta-fibrinogen gene polymorphisms
Functional sequence changes in promoter of a gene may have a direct effect on the rate of transcriptional and thus on plasma levels of the protein. Several common polymorphisms in the beta-fibrinogen gene have been identified that affect fibrinogen concentrations. Since elevated plasma fibrinogen is independent risk factor for peripheral arterial disease, these polymorphisms are of practical interest. We determined the allele distribution of two sequence variations, a G/A at position -455 and a C/T at position -148 of the beta-fibrinogen gene promoter. Genotyping was performed on whole blood DNA of 50 subjects by PCR-RFLP (Thomas et al., 1996). A fragment of 1.3 kb was amplified framing a portion of the beta fibrinogen gene from -1178 bp from the start of the transcription to +122 bp. PCR products were digested with the restriction enzymes Hae III (-455 G/A) and Hind III (-148 C/T). Genotype of each sample was determined by electrophoresis of the cleavage products on the Precast ClearoseŽ BG gels. Study results showed that same 25 (50%) individuals were homozygous for G (-455) and C (-148) alleles, 24 (48%) were heterozygous for both alleles (G/A and C/T) and 1 (2%) was homozygous for A (-455) and T (-148) alleles. Frequencies were 74 % for G and C alleles and 26 % for A and T alleles. In conclusion, distribution of allele and genotype frequencies was same for both polymorphisms. These results showed that -455 G/A polymorphism is in complete allelic association with -148 C/T polymorphism. Our findings were consistent with so far studied Caucasian populations.
PCR-RFLP; beta-fibrinogen gene polymorphisms
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nije evidentirano
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Podaci o prilogu
187-x.
2002.
objavljeno
Podaci o matičnoj publikaciji
1st Croatian Congress of Molecular Life Sciences, Book of abstracts
Dumić, Jerka et al.
Zagreb: Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu
Podaci o skupu
1st Croatian Congress of Molecular Life Sciences with international particpation
poster
09.06.2002-13.06.2002
Opatija, Hrvatska