engleski

Pathology and phylogenetic analysis of some viruses identified in wildlife birds in Croatia: Preliminary research

The article documents the results of pathomorphological and molecular diagnostic testing (polymerase chain reaction, PCR) for adenovirus, astrovirus, circovirus, hepadnavirus and coronavirus determination in a sample of 16 wildlife birds of nine various species (white stork (Ciconia ciconia), mute swan (Cygnus olor), Caspian gull (Larus cachinnans, Pallas 1811), mallard duck (Anas platyrhynchos), common buzzard (Buteo buteo), white-tailed eagle (Haliaeetus albicilla), domestic pigeon (Columba livia domestica), barn swallow (Hirundo rustica) and sparrow (Passer sp.). The birds were necropsied at the Laboratory of Pathology, Poultry Centre, Croatian Veterinary Institute, Zagreb, Croatia. Dual infections with astrovirus and circovirus were confirmed in mallard duck having died of the consequences of botulism. The sequence DuCV/M10-448 derived from the mallard duck samples had the greatest nucleotide (99%) and amino acid (100%) similarities with duck circovirus (accession number DQ100076.1) detected in the USA. The PCR positive result of hepadnavirus infection was partly confirmed by histopathologic examination in Caspian gull which suffered from generalized amyloidosis. According to the authors' awareness, this was the very first finding of hepadnavirus in gulls. The circovirus sequence found in the domestic pigeon (PiCV/M10-513) showed a 100% nucleotide and amino acid homology with pigeon circovirus (accession number AF252610.1) identified in Germany. In one of virologically tested mute swans, pathomorphological examination confirmed iron storage disease (ISD), while the rest of the birds died of the consequences of ballistic trauma or gunshot wounds (GSW) and other traumatic lesions (white stork, mute swan, common buzzard, white-tailed eagle, barn swallow and sparrow). Molecular diagnostic test results for the West Nile, avian influenza and Newcastle disease viruses were negative.

wildlife birds ; pathomorphology ; histopathology ; polymerase chain reaction ; astrovirus ; hepadnavirus ; adenovirus ; circovirus ; coronavirus ; Caspian gull (Larus cachinnans ; Pallas 1811) ; mallard duck (Anas platyrhynchos) ; domestic pigeon (Columba livia domestica) ; mute swan (Cygnus olor) ; iron storage disease (ISD) ; amyloidosis

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