engleski

Detection of mucosal human papillomavirus types in extra-genital Bowen's disease

Introduction: The association between high-risk mucosal types of human papillomavirus (HPV) and genital Bowen's disease is already well established. The aim of this study was to determine presence of high-risk mucosal human papillomavirus types in extra-genital Bowen's disease. Materials and methods: Nineteen paraffin embeded tissues histologicaly verified as Bowen's disease were analysed. All lesions were located on the head. Detection of mucosal HPV DNA was performed using HPV screening kit (AID, Germany) according to their protocol. High-risk HPV types (16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 82) were detected by polymerase chain reaction and subsequent reverse hybridisation with sequence- specific oligonucleotide probes. Results: In all specimens analysis was negative for high-risk HPV types. In five out of nineteen samples (26%) there were no control DNA and we can not claim for certain that they are negative for high-risk HPV types. Conclusion: Most former studies have emphasised the role of high- risk HPV types in extra-genital Bowen's disease, especially HPV16. Regarding our results we did not confirm connection between high- risk HPV types and extra-genital Bowen's disease. Negative results obtained in this study could be due to small sample and/or difficulties related to sampling and low number of copies of the HPV genoma.

Bowen's disease ; human papillomavirus type 16 ; human papillomavirus type 18 ; carcinoma in situ ; squamous cell carcinoma

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