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Testosterone-induced micronuclei and increased nuclear division rate in L929 cell line expressing the androgen receptor


Stojković, Ranko; Ivanković, Siniša; Ivanković, Dušica; Attias, Leonello; Mantovani, Alberto; Fučić, Aleksandra
Testosterone-induced micronuclei and increased nuclear division rate in L929 cell line expressing the androgen receptor // Toxicology in vitro, 29 (2015), 5; 1021-1025 doi:10.1016/j.tiv.2015.04.007 (međunarodna recenzija, članak, znanstveni)


Naslov
Testosterone-induced micronuclei and increased nuclear division rate in L929 cell line expressing the androgen receptor

Autori
Stojković, Ranko ; Ivanković, Siniša ; Ivanković, Dušica ; Attias, Leonello ; Mantovani, Alberto ; Fučić, Aleksandra

Izvornik
Toxicology in vitro (0887-2333) 29 (2015), 5; 1021-1025

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Testosterone; Micronucleus; Benchmark dose; L929

Sažetak
Data on the possible genotoxic effects of testosterone are limited: in particular, the potential clastogenic and/or aneugenic effects have not yet been properly investigated. An in vitro micronucleus (MN) assay was performed on L929 cells exposed to testosterone at doses of 10, 15, 20, 30, and 40 lg/mL. Significantly increased MN frequencies were detected at doses of 20, 30, and 40 lg/mL after 24 h and 48 h of incubation. The nuclear division index was higher after 48 h than 24 h of incubation. A benchmark dose (BMD) calculation was used to estimate the 1% extra risk level for MN and increased tetranucleated cells. The calculated 1% extra risk level for MN at 24 h was 12.01 lg/mL, with a 95% lower confidence limit (BMDL) at 8.98 lg/mL ; the corresponding BMD and BMDL at 48 h were 17.35 lg/mL and 10.69 lg/mL, respectively. The BMD for tetranucleated cells at 24 h was 14.86 lg/mL, with a BMDL of 7.75 lg/mL ; the corresponding values at 48 h were 0.50 lg/mL for BMD and 0.87 lg/mL for BMD. These findings suggest that the intensity of the mitogenic effect of testosterone increases upon prolonged exposure. The results of our study show that testosterone acts both as a mitogenic and genotoxic agent in L929 cells.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Ustanove
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Institut "Ruđer Bošković", Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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