engleski

mRNA isolation from archive paraffin-embedded lymphatic tissue specimens for transcription factor expression analysis

Gene activation that underlies lymphoid cell differentiation has been one of the more investigated topics in immunology in the recent years. However, the analysis of these molecular events in lymphoproliferative diseases is often hampered by the lack of fresh material. Most tissues available for routine pathological investigation are formalin-fixed and paraffin-embedded. Gene expression in such specimens could be analysed using reverse transcription of mRNA and polymerase chain reaction (RT-PCR). Therefore we established a method for mRNA isolation from such specimens by modifying the phenol/chloroform extraction method. Given the significance of transcription factors in the human hemopoietic system, we investigated whether mRNA could be successfully isolated from archival tissue, for a comprehensive study on expression of transcription factors in lymphatic tissue

RNA isolation; paraffin-embedded tissue; transcription factor

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