Naslov
Stability of Minimum Essential Media functionality despite L-glutamine decomposition

Autori
Jagušić, Maja ; Forčić, Dubravko ; Brgles, Marija ; Kutle, Leonida ; Šantak, Maja ; Jergović, Mladen ; Kotarski, Ljerka ; Bendelja, Krešo ; Halassy, Beata

Izvornik
Cytotechnology (0920-9069) 68 (2016), 4; 1171-1183

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Minimum Essential Medium (MEM); L-Glutamine; Cell viability; Apoptosis; Necrosis; Measles; Mumps; Rubella

Sažetak
L-Glutamine (L-Gln) instability in liquid media has been a well-known fact. Also, negative effect of ammonia, one of the L-Gln degradation products, on viability of many cell cultures and on different viruses’ growth has been described. However, negative effects of ammonia have been reported in doses excessively exceeding those that could be generated in regularly used liquid culture media due to L- Gln spontaneous breakdown (bellow 2 mM). Traditional virus vaccine production processes have been established and registered involving L-Gln containing media use. Eventual culture media replacement in the regular production process belongs to the major regulative changes that require substantial financial expenses. The aim of this study was to evaluate the effect of Minimum Essential Media with Hanks salts (MEM-H) storage on its relevant biological functions during virus vaccine production process, in relation to L-Gln decrease. Our results show a cell-type dependent effect of spontaneous L-Gln degradation during media storage. They also suggest that for cell cultures used in measles, mumps, and rubella virus production the media retain their functionality in respect to cell viability or virus growth over certain window of time despite L-Gln degradation.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Biotehnologija

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