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NEW KINETIC METHOD FOR DETERMINATION OF N-ACETYL-L-CYSTEINE IN PHARMACEUTICAL FORMULATIONS


Kukoč Modun, Lea; Biočić, Maja; Radić, Njegomir
NEW KINETIC METHOD FOR DETERMINATION OF N-ACETYL-L-CYSTEINE IN PHARMACEUTICAL FORMULATIONS // Prvi hrvatski kongres kliničke farmacije s međunarodnim sudjelovanjem Optimizacija farmakoterapije i sigurnost bolesnika / Bačić Vrca, Vesna ; Jakševac Mikša, Maja ; Ortner Hadžiabdić Maja, Vladimir Knežević, Sandra (ur.).
Zagreb: Hrvatsko farmaceutsko društvo, 2014. str. 84-84 (poster, međunarodna recenzija, sažetak, znanstveni)


Naslov
NEW KINETIC METHOD FOR DETERMINATION OF N-ACETYL-L-CYSTEINE IN PHARMACEUTICAL FORMULATIONS

Autori
Kukoč Modun, Lea ; Biočić, Maja ; Radić, Njegomir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Prvi hrvatski kongres kliničke farmacije s međunarodnim sudjelovanjem Optimizacija farmakoterapije i sigurnost bolesnika / Bačić Vrca, Vesna ; Jakševac Mikša, Maja ; Ortner Hadžiabdić Maja, Vladimir Knežević, Sandra - Zagreb : Hrvatsko farmaceutsko društvo, 2014, 84-84

ISBN
978-953-7897-04-8

Skup
1. Hrvatski kongres kliničke farmacije s međunarodnim sudjelovanjem

Mjesto i datum
Zagreb, Hrvatska, 10-12.04.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
N-Acetylcysteine; Kinetic spectrophotometry; pharmaceutical formulations

Sažetak
INTRODUCTION. N-acetyl-L-cysteine (NAC) is a synthetic aminothiol antioxidant that has been in clinical use for more than 40 years, primarily as a mucolytic agent and in the management of paracetamol (acetaminophen) poisoning. There is a need for a new, robust, inexpensive, and rapid method of determination of NAC in pharmaceutical formulations, in order to assure proper quality control (QC). Patient safety during therapy, on the other hand, is indirectly linked with proper QC of the medication. Novel flow-injection spectrophotometric method for the determination of NAC has been developed and validated. MATERIALS AND METHODS. The proposed method is based on the reduction of Cu(II)-neocuproine reagent to Cu(I)-neocuproine with the analyte, in a Britton-Robinson buffer solution (pH = 3.0). The non-steady state absorbance of the formed yellow Cu(I)-neocuproine complex is measured at 458 nm. The three-line manifold with one reaction coil was used. Optimization of manifold parameters and experimental conditions were carried out by means of univariate method. RESULTS. A linear calibration curve is established in a concentration range of 6×10−7 to 4×10−5 mol L−1 NAC with regression equation y = 3.89´103 –0, 0015 (R2 = 0.9996) (n = 9) and detection limit of 9.4×10−8. The proposed method is simple, rapid, sensitive and reproducible (RSD 0.9%, n = 100). In addition, proposed method is sensitive enough to enable determination of near nanomole amounts of the NAC without expensive instruments with the analytical frequency of 120 h−1. CONCLUSION. The proposed method can be applied for the determination of NAC in pharmaceutical preparations. Therefore, it could be useful for QC of NAC-containing medications, indirectly improving patient safety during therapy (reducing risk of under or over- dosage).

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekt / tema
011-0000000-3217 - Određivanje tiola primjenom potenciometrijskih senzora i spektroskopije (Njegomir Radić, )

Ustanove
Kemijsko-tehnološki fakultet, Split