Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 756387

Integrated RNA- and protein profiling of fermentation and respiration in diploid budding yeast provides insight into nutrient control of cell growth and development


Becker, E.; Liu, Y.; Lardenois, A.; Walther, T.; Horecka, J.; Stuparević, Igor; Law, M. J.; Lavigne, R.; Evrard, B.; Demougin, P. et al.
Integrated RNA- and protein profiling of fermentation and respiration in diploid budding yeast provides insight into nutrient control of cell growth and development // Journal of Proteomics, 119 (2015), 30-44 doi:10.1016/j.jprot.2015.01.015 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 756387 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Integrated RNA- and protein profiling of fermentation and respiration in diploid budding yeast provides insight into nutrient control of cell growth and development

Autori
Becker, E. ; Liu, Y. ; Lardenois, A. ; Walther, T. ; Horecka, J. ; Stuparević, Igor ; Law, M. J. ; Lavigne, R. ; Evrard, B. ; Demougin, P. ; Riffle, M. ; Strich, R. ; Davis, R. W. ; Pineau, C. ; Primig, M.

Izvornik
Journal of Proteomics (1874-3919) 119 (2015); 30-44

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
fermentation; proteome; respiration; Rim4; transcriptome; Ume6

Sažetak
Diploid budding yeast undergoes rapid mitosis when it ferments glucose, and in the presence of a non-fermentable carbon source and the absence of a nitrogen source it triggers sporulation. Rich medium with acetate is a commonly used pre-sporulation medium, but our understanding of the molecular events underlying the acetate-driven transition from mitosis to meiosis is still incomplete. We identified 263 proteins for which mRNA and protein synthesis are linked or uncoupled in fermenting and respiring cells. Using motif predictions, interaction data and RNA profiling we find among them 28 likely targets for Ume6, a subunit of the conserved Rpd3/Sin3 histone deacetylase-complex regulating genes involved in metabolism, stress response and meiosis. Finally, we identify 14 genes for which both RNA and proteins are detected exclusively in respiring cells but not in fermenting cells in our sample set, including CSM4, SPR1, SPS4 and RIM4, which were thought to be meiosis-specific. Our work reveals intertwined transcriptional and post-transcriptional control mechanisms acting when a MATa/α strain responds to nutritional signals, and provides molecular clues how the carbon source primes yeast cells for entering meiosis. BIOLOGICAL SIGNIFICANCE: Our integrated genomics study provides insight into the interplay between the transcriptome and the proteome in diploid yeast cells undergoing vegetative growth in the presence of glucose (fermentation) or acetate (respiration). Furthermore, it reveals novel target genes involved in these processes for Ume6, the DNA binding subunit of the conserved histone deacetylase Rpd3 and the co-repressor Sin3. We have combined data from an RNA profiling experiment using tiling arrays that cover the entire yeast genome, and a large-scale protein detection analysis based on mass spectrometry in diploid MATa/α cells. This distinguishes our study from most others in the field-which investigate haploid yeast strains-because only diploid cells can undergo meiotic development in the simultaneous absence of a non-fermentable carbon source and nitrogen. Indeed, we report molecular clues how respiration of acetate might prime diploid cells for efficient spore formation, a phenomenon that is well known but poorly understood.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Biotehnologija



POVEZANOST RADA


Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb

Profili:

Avatar Url Igor Stuparević (autor)

Citiraj ovu publikaciju:

Becker, E.; Liu, Y.; Lardenois, A.; Walther, T.; Horecka, J.; Stuparević, Igor; Law, M. J.; Lavigne, R.; Evrard, B.; Demougin, P. et al.
Integrated RNA- and protein profiling of fermentation and respiration in diploid budding yeast provides insight into nutrient control of cell growth and development // Journal of Proteomics, 119 (2015), 30-44 doi:10.1016/j.jprot.2015.01.015 (međunarodna recenzija, članak, znanstveni)
Becker, E., Liu, Y., Lardenois, A., Walther, T., Horecka, J., Stuparević, I., Law, M., Lavigne, R., Evrard, B. & Demougin, P. (2015) Integrated RNA- and protein profiling of fermentation and respiration in diploid budding yeast provides insight into nutrient control of cell growth and development. Journal of Proteomics, 119, 30-44 doi:10.1016/j.jprot.2015.01.015.
@article{article, year = {2015}, pages = {30-44}, DOI = {10.1016/j.jprot.2015.01.015}, keywords = {fermentation, proteome, respiration, Rim4, transcriptome, Ume6}, journal = {Journal of Proteomics}, doi = {10.1016/j.jprot.2015.01.015}, volume = {119}, issn = {1874-3919}, title = {Integrated RNA- and protein profiling of fermentation and respiration in diploid budding yeast provides insight into nutrient control of cell growth and development}, keyword = {fermentation, proteome, respiration, Rim4, transcriptome, Ume6} }
@article{article, year = {2015}, pages = {30-44}, DOI = {10.1016/j.jprot.2015.01.015}, keywords = {fermentation, proteome, respiration, Rim4, transcriptome, Ume6}, journal = {Journal of Proteomics}, doi = {10.1016/j.jprot.2015.01.015}, volume = {119}, issn = {1874-3919}, title = {Integrated RNA- and protein profiling of fermentation and respiration in diploid budding yeast provides insight into nutrient control of cell growth and development}, keyword = {fermentation, proteome, respiration, Rim4, transcriptome, Ume6} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati:





    Contrast
    Increase Font
    Decrease Font
    Dyslexic Font