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Pregled bibliografske jedinice broj: 75409

Polymerase chain reaction (PCR) based detection of Streptococcus mitis and Leptotrichia buccalis from periodontal pockets


Jurak, Igor; Ivić-Kardum, Marija; Pavelić, Jasminka; Gall-Trošelj, Koraljka
Polymerase chain reaction (PCR) based detection of Streptococcus mitis and Leptotrichia buccalis from periodontal pockets // Periodicum Biologorum, 103 (2001), 2; 175-178 (međunarodna recenzija, članak, znanstveni)


Naslov
Polymerase chain reaction (PCR) based detection of Streptococcus mitis and Leptotrichia buccalis from periodontal pockets

Autori
Jurak, Igor ; Ivić-Kardum, Marija ; Pavelić, Jasminka ; Gall-Trošelj, Koraljka

Izvornik
Periodicum Biologorum (0031-5362) 103 (2001), 2; 175-178

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Periodonttis; PCR; Streptococcus mitis; Leptotrichia buccalis

Sažetak
Background and Purpose: Periodontal diseases are infectious diseases followed by inflammatory changes in periodontal tissues, which lead to destruction of periodontal tissue and tooth loss. Many risk factors other than bacterial infection are involved in the development and progression of the disease: e.g. stress and cigarette smoking. The aim of this study was to establish a highly specific polymerase chain reaction (PCR), assay for detection of two strains of bacteria, Streptococcus mitis and Leptotrichia buccalis, which are putative periodontal pathogens. Materials and Methods: Paper points were immersed in periodontal pockets of patients with periodontal disease in order to collect bacteria, followed by DNA extraction and PCR, which was performed with primers constructed according to published bacterial DNA sequences from the Gene Bank. The specificity of positive samples was further confirmed by cutting the amplicon with restriction endonucleases (RFLP; restriction fragment length polymorphism). results and Conclusions: the primers specific for detection of Streptococcus mitis and Leptotrichia buccalis were constructed and optimized for PCR assays. The high specificity of expected PCR amplicons was confirmed by RFLP method. Hence, we strongly recommend the PCR assay described herein for specific detection of Streptococcus mitis and Leptotrichia buccalis in periodontal pockets.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
00981103

Ustanove
Institut "Ruđer Bošković", Zagreb

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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