engleski

Simultaneous plasmid integration: a unifying model of multiple plasmid integration into the yeast chromosome

Recombination of non-replicative plasmids bearing yeast homology with the chromosome can integrate the plasmid molecule into the genome. Such process is also known to integrate more than one plasmid molecule leading to multiple, tandem plasmid integration. However, its exact molecular mechanism remains unknown. There are two alternative models to explain such integration. The first predicts single integration of a super-plasmid molecule and the second sequential integration of several independent molecules, but neither is able to comprehend all experimental data. Therefore, here is presented a theoretical model that unifies both prior models owing to the possibility that two plasmid molecules recombine with the chromosome simultaneously. This model was used as a theoretical tool in order to discriminate between existing alternatives extracting the sequential model as a better overall explanation.

Saccharomyces; homologous recombination; multiple plasmid integration; genetic model

nije evidentirano