engleski

Molecular characterization of 'Plavac mali' and 'Sangiovese' berry color mutants

Due to vegetative propagation grapevine cultivars are supposed to be genetically identical offspring of a parent seedling. However, during time spontaneous mutations occur within the populations of cultivars resulting in vines with new traits. This phenomenon has been utilized in selection by humans to establish subpopulations with new agronomic value, usually called clones. Clones of a variety often have unique and superior traits. Mutations in grape berry skin are known and found in several cultivars (e.g. Pinot family). Color of the berry skin in grapevine is dependent on the presence or absence of anthocyanin. Anthocyanin biosynthesis is mostly controlled with a cluster of Myb-type genes. ‘Plavac mali’ is a major Croatian red cultivar and ‘Sangiovese’ is the most widely planted red cultivar in Italy. Color mutants of these varieties have been found: pink-berried mutant of ‘Plavac mali’, also called ‘Plavac mali sivi’ and several bud sports of ‘Sangiovese’. In this study, layer specific molecular characterization have been performed. Leaf and root DNA samples have been used to test if the mutations are chimeric. Genome region along chromosome 2 containing Myb genes was analyzed using previously known and newly developed SSR markers. Applied markers enabled differentiation of mutants and wild type in both cultivars but in spite of very similar phenotype genetic background was different.

berry color; bud sports; MYB genes; SSRs; SNPs

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