engleski

CADMIUM-TRIGGERED CALLOSE DEPOSITION IN TWO MAIZE GENOTYPES

Cadmum is a non-essential metal and an environmental hazard due to its toxicity to plants and animals. Callose is a polysaccharide that plays an important role in plant development and in response to various stresses. Seeds of two maize genotypes Os6-2 inbred line and OS 602 hybrid were sown in plastic pots filled with two types of soil: control – untreated eutric cambisol and cadmium contaminated soil – eutric cambisol treated with CdCl2 solution up to concentration of 5 mg Cd/ kg soil. Approximately 5 mm segments above adventitious roots of control and cadmium treated 10 days old seedlings were cut and a wet mount slides were prepared. Mounts were stained with 0.005% aniline blue solution. Callose was detected under a fluorescent microscope. Results of this preliminary study show that it is likely that cadmium is another metal besides aluminum that causes callose deposition in maize cell walls with no apparent differences between these two genotypes.

maize; cadmium toxicity; callose

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