engleski

Treatments of acariasis with ivermectin and evaluation of different sampling techniques in mice

Breeding mice positive for Myocoptes musculinus and Myobia musculi were treated with ivermectin in doses of 200 µg7kg of body weight (6 µl of active substance) as a spot application on the back of the neck. Application was repeated three times in seven-day intervals. Different development forms (adults, nymphs, larvae, and eggs) were observed in groups of adult and young mice before the treatment as well as during and after the treatment. Before treatment, the fur pluck technique and sticky paper technique (sampling from two different places: from back and abdomen) were evaluated. The fur pluck technique was used as the gold standard. The sticky paper technique has 91.5 to 93.2 % sensitivity for Myocoptes musculinus and 10.8 to 13.5% for Myobia musculi. We observed that Myobia musculi place eggs next to the skin, on the bases of two or more hairs together ; consequently, the sampling is not always satisfactory. The effectiveness of therapy with ivermectin for Myocoptes musculinus and Myobia musculi was shown after the second treatment. Before the treatment, as well as during and after the treatment, a significantly higher percentage of positive mice was observed among old than in young ones. After the third treatment, adults and developmental stages (eggs, larvae, and nymphs) were still found only in Myocoptes musculinus. Almost all eggs of Myocoptes musculinus and Myobia musculi were drained and damaged after the third treatment.

veterinary science; mice; ectoparasites; Myocoptes musculinus; Myobia musculi; treatment

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