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Characterization of spiral ganglion neurons cultured on silicon micro-pillar substrates for new auditory neuro-electronic interfaces (CROSBI ID 215855)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Mattotti, Marta ; Micholt, Liesbeth ; Braeken Dries ; Kovačić, Damir Characterization of spiral ganglion neurons cultured on silicon micro-pillar substrates for new auditory neuro-electronic interfaces // Journal of neural engineering, 12 (2015), 2; 026001, 12. doi: 10.1088/1741-2560/12/2/026001

Podaci o odgovornosti

Mattotti, Marta ; Micholt, Liesbeth ; Braeken Dries ; Kovačić, Damir

engleski

Characterization of spiral ganglion neurons cultured on silicon micro-pillar substrates for new auditory neuro-electronic interfaces

Objective. One of the strategies to improve cochlear implant technology is to increase the number of electrodes in the neuro- electronic interface. The objective was to characterize in vitro cultures of spiral ganglion neurons (SGN) cultured on surfaces of novel silicon micro- pillar substrates (MPS). Approach. SGN from P5 rat pups were cultured on MPS with different micro-pillar widths (1 – 5.6 μm) and spacings (0.6 – 15 μm) and were compared with control SGN cultures on glass coverslips by immunocytochemistry and scanning electron microscopy (SEM). Main results. Overall, MPS support SGN growth equally well as the control glass surfaces. Micro- pillars of a particular size-range (1.2 – 2.4 μ m) were optimal in promoting SGN presence, neurite growth and alignment. On this speci fi c micro-pillar size, more SGN were present, and neurites were longer and more aligned. SEM pictures highlight how cells on micro-pillars with smaller spacings grow directly on top of pillars, while at wider spacings (from 3.2 to 15 μm) they grow on the bottom of the surface, losing contact guidance. Further, we found that MPS encourage more monopolar and bipolar SGN morphologies compared to the control condition. Finally, MPS induce longest neurite growth with minimal interaction of S100+ glial cells. Significance. These results indicate that silicon micro- pillar substrates create a permissive environment for the growth of primary auditory neurons promoting neurite sprouting and are a promising technology for future high-density three-dimensional CMOS-based auditory neuro- electronic interfaces.

neuro-electronic interface ; spiral ganglion neurons ; micro-pillars ; in vitro ; surface topography ; medical bionics ; cochlear implant

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Podaci o izdanju

12 (2)

2015.

026001

12

objavljeno

1741-2560

1741-2552

10.1088/1741-2560/12/2/026001

Povezanost rada

Elektrotehnika, Fizika, Temeljne medicinske znanosti

Poveznice
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