engleski

Age- and sex-dependent oxidant and antioxidant status of CBA mice

Despite of numerous reports of lipid peroxidation and antioxidant enzyme status in mice, the results are still conflicting because most of the studies were not comprehensive concerning strain, sex and age of mice. In our study of oxidative and antioxidative status, we defined the dynamics regarding to age and sex in male and female CBA mice. We did this by determining thiobarbituric acid-reactive substances (TBARS) content, superoxide dismutase (SOD) and catalase (CAT) activity in liver of 1, 4 and 10 months old male and female CBA mice. TBARS content was assayed according to Ohkawa’s method (Ohkawa et al. 1979.) ; SOD activity was assayed according to Flohé’s method (Flohé et al. 1984.) and CAT activity according to Ou’s method (Ou et al. 1995.). TBARS content significantly increased with age (P<0.001) to the same extent in both sexes. SOD activity decreased only in male mice (4 months old, P<0.001) whereas it remained unchanged in female mice. The changes observed in CAT activity were age- and sex-related ; increased in 4 and 10 months old male mice (P<0.001), and decreased in 4 months old female mice (P<0.05). Thus, it seems that changes in oxidative status are rather age- than sex-related, whereas antioxidative enzyme activities are age- and sex-related. By definition of these parameters, we can more reliable investigate the effect of various therapeutic agents on these features.

age; sex; CBA mice

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