engleski

Age- and sex-dependent oxidant and antioxidant status of CBA mice

Despite of numerous reports of lipid peroxidation and antioxidant enzyme status in mice, the results are still conflicting because most of the studies were not comprehensive concerning strain, sex and age of mice. In our study of oxidative and antioxidative status, we defined the dynamics regarding to age and sex in male and female CBA mice. By definition of these parameters, we can more reliable investigate the effect of various therapeutic agents on these features. We defined the dynamics of oxidant and antioxidant status by determining thiobarbituric acid-reactive substances (TBARS) content, superoxide dismutase (SOD) and catalase (CAT) activity in liver of 1, 4, 10 and 18 months old male and female CBA mice. TBARS content was assayed according to Ohkawa’s method (Ohkawa et al. 1979.) ; SOD activity was assayed according to Flohé’s method (Flohé et al. 1984.) and CAT activity according to Ou’s method (Ou et al. 1995.). TBARS content significantly increased with age (P<0.01) to the same extent in both sexes. With no significant difference between sexes of the same age group. SOD activity varied with age in both sexes, significantly decreasing in young male mice (4 months old, P<0.01) and remaining unchanged in female mice until 18 months of age, when it markedly decreased (p<0.01). The changes observed in CAT activity were age- and sex-related ; while in males CAT activity continuously increased with age (P<0.01), in females it decreased at 4 and increased at 18 months of age (P<0.01). Thus, it seems that changes in oxidative status are rather age- than sex-related, whereas antioxidative enzyme activities are age- and sex-related.

age; sex; oxidant/antioxidant status

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