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Pregled bibliografske jedinice broj: 74225

Effects of various cryopreservation media and freezing-thawing on the morphology of the rat testicular biopsy


Ježek, Davor; Schulze, Wolfgang; Kalanj-Bognar, Svjetlana; Vukelić, Željka; Milavec-Puretić, Višnja; Krhen, Ivan
Effects of various cryopreservation media and freezing-thawing on the morphology of the rat testicular biopsy // Andrologia, 33 (2001), 6; 368-378 (međunarodna recenzija, članak, znanstveni)


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Naslov
Effects of various cryopreservation media and freezing-thawing on the morphology of the rat testicular biopsy

Autori
Ježek, Davor ; Schulze, Wolfgang ; Kalanj-Bognar, Svjetlana ; Vukelić, Željka ; Milavec-Puretić, Višnja ; Krhen, Ivan

Izvornik
Andrologia 33 (2001), 6; 368-378

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Biopsy; Cryopreservation; Morphology; Rat; Testis

Sažetak
Currently, testicular sperm extraction is successfully combined with intracytoplasmic sperm injection into the oocyte (ICSI). Several pieces of a testicular biopsy can be frozen and thawed until the ICSI atempt. In this study, tthe effects of freezing-thawing on the morphology of rat testicular biopsies stored in different cryopreservation media were analysed. Each cryopreservation medium contained glycerol and/or dimethyl sulfoxide (DMSO) as cryopreotectants. In general, both glycerol and DMSO, when applied at moderat concentrations (6-25%), preserved the structure of the seminiferous epithelium. The freezing-thawing procedure had no significant effect on tubular diameter; however, it caused a "folding" of the lamina propria and notable damage to Sertoli cells, spermatogonia and spermatocytes. Round and elongated spermatids and spermatozoa displayed occasional nuclear damage, vacuolization, and shirnage/swelling of the cytoplasm. However, the vas majority of these cells maintained their normal structure in nearly all the applied cryomedia. It is concluded that freezing-thawing of testicular biopsies, and the cryopreservation medium, have a significant impact on the structure of the seminiferous epithelium, particularly ont its basal compartment.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
108121

Ustanove:
Medicinski fakultet, Zagreb

Citiraj ovu publikaciju

Ježek, Davor; Schulze, Wolfgang; Kalanj-Bognar, Svjetlana; Vukelić, Željka; Milavec-Puretić, Višnja; Krhen, Ivan
Effects of various cryopreservation media and freezing-thawing on the morphology of the rat testicular biopsy // Andrologia, 33 (2001), 6; 368-378 (međunarodna recenzija, članak, znanstveni)
Ježek, D., Schulze, W., Kalanj-Bognar, S., Vukelić, Ž., Milavec-Puretić, V. & Krhen, I. (2001) Effects of various cryopreservation media and freezing-thawing on the morphology of the rat testicular biopsy. Andrologia, 33 (6), 368-378.
@article{article, year = {2001}, pages = {368-378}, keywords = {Biopsy, Cryopreservation, Morphology, Rat, Testis}, journal = {Andrologia}, volume = {33}, number = {6}, title = {Effects of various cryopreservation media and freezing-thawing on the morphology of the rat testicular biopsy}, keyword = {Biopsy, Cryopreservation, Morphology, Rat, Testis} }
@article{article, year = {2001}, pages = {368-378}, keywords = {Biopsy, Cryopreservation, Morphology, Rat, Testis}, journal = {Andrologia}, volume = {33}, number = {6}, title = {Effects of various cryopreservation media and freezing-thawing on the morphology of the rat testicular biopsy}, keyword = {Biopsy, Cryopreservation, Morphology, Rat, Testis} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • SCI-EXP, SSCI i/ili A&HCI





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