engleski

Reg genes expression in regenerating skeletal muscle

The Reg genes have been documented to play an important role in various tissue regeneration processes, cell proliferation, differentiation and carcinogenesis. Until now, few extensive analyses have been made of Reg gene expression changes in rat skeletal muscles after injury. The aim of this investigation was to reveal the transcriptional activation of Reg genes in the skeletal muscles in response to injury. In 2-month-old Wistar rats we used three experimental models of muscle and nerve injury. The regeneration process was induced in both type of muscles, slow (m.soleus, SOL) and fast (m. extensor digitorum longus, EDL) by injection of 1 ml of local anesthetic, by denervation and combined model of muscle denervation and injection of local anesthetic. The RT-PCR analysis was performed for detection of Reg1, Reg3G and Reg4 mRNA in regenerating muscles. RT-PCR analysis showed that Reg1 gene is expressed in EDL and SOL with peak expression 12 hours after injury and signal intensity was constant till 7th day in all three experimental models. Reg 3G can be detected over a longer period in comparison with Reg1 and signal intensity was lower. There was no detectable Reg4 mRNA expression in either SOL or EDL muscle. Our investigation revealed presence of Reg1 and Reg3G genes in regenerating skeletal muscle and its expression is up regulated with regeneration process. Reg1 and Reg3G genes expression was induced in both skeletal muscles at a considerably early time point after muscle injury. Our results imply that Reg genes have a significant role at the early stage of skeletal muscle regeneration.

reg genes; regeneration; skeletal muscle

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