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The study of the interactions of 1, 2-dimyristoyl-sn-glycero3-phosphocholine with myricetin by IR spectroscopy

Myricetin (MY) is a herbal compound that belongs to the class of flavonols and expresses various beneficial effects for animal organisms [1]. As the mechanism of its actions is still vague, its elucidation primary requires the research of its pharmacological activities. It is essential to explore MY interactions with biological and, very often, model membranes [2]. In this work, the interactions between 1, 2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) as model lipid and MY are explored by using IR spectroscopy. Pure DMPC and the mixtures of DMPC and MY, containing various concentrations of MY, were recorded as liposomes in phosphate buffer (PBS). From the position of the band attributed to the symmetric CH2 stretching, DMPC melting temperature (Tm) was determined. It is 24 °C for pure DMPC, while for DMPC+MY mixtures, Tm decreases with MY concentration. For molar ratio n(DMPC):n(MY)=1: 0.35, Tm is 19 °C . Due to the lowering of melting point of DMPC in the presence of MY, the incorporation of the latter is unambigously proved. Their interaction pattern, obtained by recording multilayered films of DMPC and MY, hydrated with PBS, suggest the preferential associations of MY with the polar-apolar interface of DMPC and, respectively, with its polar head groups. The findings obtained here will serve as the platform for the study of MY effect on the neural cells [3] and, hopefully, will provide an insight into its regenerating ability. [1] Y. S. Tarahovsky et al., Biochim. Biophys. Acta, 2014, 1838, 1235. [2] M. Arczewska et al., Biochim. Biophys. Acta, 2013, 1828, 213. [3] M. Jazvinšćak Jembrek et al., J. Mol. Neurosci., 2012, 47, 286.

1; 2-dimyristoyl-sn-glycero-3-phosphocholine; myricetin; IR spectroscopy; phase transitions

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