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Pregled bibliografske jedinice broj: 741015

Sarcoplasmic reticulum Ca2+-ATPase from rabbit skeletal muscle modified by peroxynitrite


Žižková, P.; Viskupičová, J.; Blaškovič, Dušan; Štrosová, M.K.; Žarković, Neven; Horáková, L.
Sarcoplasmic reticulum Ca2+-ATPase from rabbit skeletal muscle modified by peroxynitrite // Journal of enzyme inhibition and medicinal chemistry, 29 (2014), 4; 563-570 doi:10.3109/14756366.2013.827676 (međunarodna recenzija, članak, znanstveni)


Naslov
Sarcoplasmic reticulum Ca2+-ATPase from rabbit skeletal muscle modified by peroxynitrite

Autori
Žižková, P. ; Viskupičová, J. ; Blaškovič, Dušan ; Štrosová, M.K. ; Žarković, Neven ; Horáková, L.

Izvornik
Journal of enzyme inhibition and medicinal chemistry (1475-6366) 29 (2014), 4; 563-570

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
4-Hydroxynonenal; calcium pump; carbonyls; oxidative stress

Sažetak
Effect of peroxynitrite on SERCA1 activity was studied in correlation with enzyme carbonylation. Kinetic parameters and location of peroxynitrite effect on SERCA1 were determined. Carbonyls were determined by immunoblotting. FITC, NCD-4 and Trp fluorescence were used to indicate changes in cytosolic and transmembrane regions of SERCA1. Peroxynitrite-concentration-dependent decrease of SERCA1 activity was associated with elevation of protein carbonyls. 4-HNE was not involved in carbonylation of SERCA1. Increased FITC fluorescence in the presence of peroxynitrite correlated with the decrease of the enzyme affinity to ATP. Peroxynitrite-induced SERCA1 carbonylation that was not accompanied with the formation of 4-HNE-SERCA1 adducts is indicative of direct oxidation of SERCA1. As assessed by FITC fluorescence and decreased affinity of the enzyme to ATP, peroxynitrite impairment was found to occur in the cytosolic ATP-binding region of SERCA1.

Izvorni jezik
Engleski



POVEZANOST RADA


Projekt / tema
098-0982464-2519 - Lipidi, slobodni radikali i njihovi glasnici u integrativnoj onkologiji (Neven Žarković, )
108-0000000-0028 - Oksidacijski stres i tumori središnjeg živčanog sustava (Kamelija Žarković, )

Ustanove
Institut "Ruđer Bošković", Zagreb,
Medicinski fakultet, Zagreb

Autor s matičnim brojem:
Neven Žarković, (126952)

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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