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Pregled bibliografske jedinice broj: 74048

The chloroplast protein import receptors Toc34 and Toc 195 are phosphorylated by distinct protein kinases


Fulgosi, Hrvoje; Soll, Juergen
The chloroplast protein import receptors Toc34 and Toc 195 are phosphorylated by distinct protein kinases // Journal of Biological Chemistry, 277 (2002), 11; 8934-8940 (međunarodna recenzija, članak, znanstveni)


Naslov
The chloroplast protein import receptors Toc34 and Toc 195 are phosphorylated by distinct protein kinases

Autori
Fulgosi, Hrvoje ; Soll, Juergen

Izvornik
Journal of Biological Chemistry (0021-9258) 277 (2002), 11; 8934-8940

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Protein translocation; phosphorylation; preproteins

Sažetak
The molecular composition of chloroplast outer and inner envelope translocons is fairly well established, but little is known about mechanisms and elements involved in import regulation. After synthesis in the cytosol chloroplast targeted precursor proteins are recognized by outer envelope receptors Toc34 and Toc159. Phosphorylation plays an important role in regulation of Toc34 activity and preprotein binding. Using kinase renaturation assays we have identified an ATP-dependent 98 kDa outer envelope kinase which is able to selectively phosphorylate Toc34 at a specific site. A 70 kDa outer envelope polypeptide phosphorylating Toc159 was identified by the same strategy. Antiserum against the 98 kDa kinase inhibits phosphorylation of Toc34 while labelling of Toc159 remains unaffected. Both kinases do not autophosphorylate in vitro and are unable to utilise myelin basic protein as substrate. We propose that distinct kinases are involved in regulation of chloroplast import via desensitization of preprotein receptors.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekt / tema
00981005

Ustanove
Institut "Ruđer Bošković", Zagreb

Autor s matičnim brojem:
Hrvoje Fulgosi, (186771)

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • Biological Abstracts