engleski

Modification of comet-FISH technique by using temperature instead of chemical denaturation

Comet-FISH technique is an extension of commonly used comet assay.Its purpose is to determine whether primary DNA damage which comet assay detects occurred within a sequence of interest that is visualized by hybridization of fluorescent probe. Presence of the signal in comet tail indicates impaired structural integrity of sequence. Our modifications to the original comet-FISH technique described by Rappet al.(2000) [1] include:  increase in probe binding specificity,  increased rate of successful hybridization,  simultaneous temperature denaturation of both, slide and probe.

Comet-FISH; TP53; Temperature denaturation; Lymphocytes; Primary DNA damage; Gene fragmentation

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