engleski

Molecular diagnosis of oral infections

Mnogi virusi, gljive i bakterije nastanjuju usnu šupljinu. Dok se većina tih mikroorganizama može lako uzgojiti i identificirati klasičnim mikrobiološkim testovima, neki od njih se mogu identificirati samo metodama molekularne biologije, kako npr. humani papiloma virus. Neki mikroorganizmi su prisutni u premaloj količini pa se ne mogu utvrditi klasičnim metodama tako da i u tom slučaju molekularna dijagnostika postaje logična alternativa. Testovi molekularne biologije osjetljivi su, objektivni, lako izvodljivi i manje su zahtjevni od klasičnih mikrobioloških metoda, u smislu edukacije i osiguranja kvalitete. U ovom radu su opisane molekularne metode koje se najčešće koriste za dokazivanje mikroorganizama koji koloniziraju usnu šupljinu.

virus ; fungi ; bacteria ; mouth ; diagnostics

Many viruses, fungi and bacteria inhabit the mouth. Most of these microorganisms can be easily cultured and identified by classical microbiological tests, but some of them, such as human papilloma virus, can only be identified by the methods of molecular biology. Some microorganisms are present in a very small amount and cannot be detected by classical tests ; therefore, in this case, molecular diagnosis is also a logical alternative. Molecular biology assays are sensitive, objective, easy to perform and less demanding than conventional microbiological methods in terms of training and quality assurance. This paper describes the molecular methods that are commonly used for the detection of microorganisms that colonize the oral cavity.