engleski

Don’t clean the tendon from muscle tissue in ACL reconstruction

Tendon to bone healing within the tunnels in ACL reconstruction can be enhanced by muscle tissue which is rich with mesenchymal stem cells (MSC).The purpose of this research is to enable and stimulate differentiation of the MSCs isolated from the muscles and tendons harvested as an autograft. Further objective is to enhance tendon ingrowths in the bone tunnels with the use of MSCs gained from muscle tissue that is usually treated as waste after harvesting. At the time of surgery both tendons are separated from residual muscle tissue and sized to 22 cm in length. The remaining length of the muscles is being processed within the study. Four study groups are formed: gracilis muscle tissue, gracilis tendon tissue, semitendinosus muscle tissue and semitendinosus tendon tissue. Osteogenic potential of both tissues (muscle and tendon) was measured first day after harvesting and 14 days afterwards. Tissue cultures were stained histochemically for ALP and mineralized matrix was visualized with von Koss method. Activity of ALP was measured 14 days after beginning of differentiation of mesenchymal stem cells isolated from semitendinosus and gracilis muscles, semitendinosus and gracilis tendons. Purple intense color was gained in cells differed from muscle tissue which shows significantly larger activity of ALP comparing to the cells differed from tendons. Visualization of mineralized matrix with von Koss citochemic method showed more intense black color gained in cells differed from muscle tissue comparing to the differed from tendons.The obtained results will be tested in an animal study and then clinically.

ACL reconstruction; tendon; muscle; MSCs

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