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Pregled bibliografske jedinice broj: 738960

Suppression of Smad-1 mRNA expression level by Smad-2 likely control dichotomy of NF-κB and Smads mediated activation


Slade, Neda; Zorić, Arijana; Horvat, Branka; Vukšić, Mario; Kostović, Ivica; Poljak, Ljiljana
Suppression of Smad-1 mRNA expression level by Smad-2 likely control dichotomy of NF-κB and Smads mediated activation // Immunobiology, 220 (2015), 1; 48-53 doi:10.1016/j.imbio.2014.09.003 (međunarodna recenzija, članak, znanstveni)


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Naslov
Suppression of Smad-1 mRNA expression level by Smad-2 likely control dichotomy of NF-κB and Smads mediated activation

Autori
Slade, Neda ; Zorić, Arijana ; Horvat, Branka ; Vukšić, Mario ; Kostović, Ivica ; Poljak, Ljiljana

Izvornik
Immunobiology (0171-2985) 220 (2015), 1; 48-53

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Jak-1 signaling; NF-κB transcription factor; Smads mediators

Sažetak
The aim of this study was to find out how NF-κB and Smad-mediated signaling influenced the expression of astrogliogenic versus neurogenic markers of brain development in U4C cells which were either enriched (Tg Jak-1) or deprived in Jak-1 molecule (Jak-1 KO). Genetically modified U4C cells were transfected with NF-kB reporter plasmid in order to follow its activation when cells were cotransfected with different combinations of Smads constructs. In wild type cells no significant activation of NF-κB was observed while genetically modified cells exhibited somewhat different pattern of NF-κB activation depending on the Smad constructs combination used. The absence of NF-κB activation in Jak-1 transgenic cells transfected with Smad-1 plus Smad-3 was accompanied by the appearance of apoptotic cells as revealed by DAPI staining. Smad-1 expression was undetectable in Jak-1 transgenic cells and was downregulated in wild type cells upon transfection with Smad-2. The absence of p65 nuclear translocation in Smad-2 transfected cells and the presence of Smad-4 in nucleus of the same cells indicates dichotomy in NF-κB and Smads mediated signaling pathways. The significance of this study is that helps to elucidate the point of collaboration among three different signaling pathways - Jak-1 mediated cytokine signaling, NF-κB and Smads mediated pathways.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
0108000
098-0982464-2391 - Uloga mreže proteina p53/p73 u sarkomima mekih tkiva čovjeka (Slade, Neda, MZOS ) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb,
Medicinski fakultet, Zagreb

Profili:

Avatar Url Ljiljana Poljak (autor)

Avatar Url Mario Vukšić (autor)

Avatar Url Neda Slade (autor)

Avatar Url Ivica Kostović (autor)

Avatar Url Arijana Zorić (autor)

Avatar Url Branka Horvat (autor)

Citiraj ovu publikaciju

Slade, Neda; Zorić, Arijana; Horvat, Branka; Vukšić, Mario; Kostović, Ivica; Poljak, Ljiljana
Suppression of Smad-1 mRNA expression level by Smad-2 likely control dichotomy of NF-κB and Smads mediated activation // Immunobiology, 220 (2015), 1; 48-53 doi:10.1016/j.imbio.2014.09.003 (međunarodna recenzija, članak, znanstveni)
Slade, N., Zorić, A., Horvat, B., Vukšić, M., Kostović, I. & Poljak, L. (2015) Suppression of Smad-1 mRNA expression level by Smad-2 likely control dichotomy of NF-κB and Smads mediated activation. Immunobiology, 220 (1), 48-53 doi:10.1016/j.imbio.2014.09.003.
@article{article, year = {2015}, pages = {48-53}, DOI = {10.1016/j.imbio.2014.09.003}, keywords = {Jak-1 signaling, NF-κB transcription factor, Smads mediators}, journal = {Immunobiology}, doi = {10.1016/j.imbio.2014.09.003}, volume = {220}, number = {1}, issn = {0171-2985}, title = {Suppression of Smad-1 mRNA expression level by Smad-2 likely control dichotomy of NF-κB and Smads mediated activation}, keyword = {Jak-1 signaling, NF-κB transcription factor, Smads mediators} }
@article{article, year = {2015}, pages = {48-53}, DOI = {10.1016/j.imbio.2014.09.003}, keywords = {Jak-1 signaling, NF-κB transcription factor, Smads mediators}, journal = {Immunobiology}, doi = {10.1016/j.imbio.2014.09.003}, volume = {220}, number = {1}, issn = {0171-2985}, title = {Suppression of Smad-1 mRNA expression level by Smad-2 likely control dichotomy of NF-κB and Smads mediated activation}, keyword = {Jak-1 signaling, NF-κB transcription factor, Smads mediators} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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