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Ionic strength and composition govern the elasticity of biological membranes. A study of model DMPC bilayers by force- and transmission IR spectroscopy


Šegota, Suzana; Vojta, Danijela; Pletikapić, Galja; Baranović, Goran
Ionic strength and composition govern the elasticity of biological membranes. A study of model DMPC bilayers by force- and transmission IR spectroscopy // Chemistry and physics of lipids, 186 (2015), 17-29 doi:10.1016/j.chemphyslip.2014.11.001 (međunarodna recenzija, članak, znanstveni)


Naslov
Ionic strength and composition govern the elasticity of biological membranes. A study of model DMPC bilayers by force- and transmission IR spectroscopy

Autori
Šegota, Suzana ; Vojta, Danijela ; Pletikapić, Galja ; Baranović, Goran

Izvornik
Chemistry and physics of lipids (0009-3084) 186 (2015); 17-29

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
AFM; Force spectroscopy; Ion binding; Ion mixture; IR spectroscopy; Lipid bilayers; Nanomechanics; Seawater

Sažetak
Infrared (IR) spectroscopy was used to quantify the ion mixture effect of seawater (SW), particularly the contribution of Mg2+ and Ca2+ as dominant divalent cations, on the thermotropic phase behaviour of 1, 2-dimyristoyl-sn-glycero-3-posphocholine (DMPC) bilayers. The changed character of the main transition at 24 °C from sharp to gradual in films and the 1 °C shift of the main transition temperature in dispersions reflect the interactions of lipid headgroups with the ions in SW. Force spectroscopy was used to quantify the nanomechanical hardness of a DMPC supported lipid bilayer (SLB). Considering the electrostatic and ion binding equilibrium contributions while systematically probing the SLB in various salt solutions, we showed that ionic strength had a decisive influence on its nanomechanics. The mechanical hardness of DMPC SLBs in the liquid crystalline phase linearly increases with the increasing fraction of all ion-bound lipids in a series of monovalent salt solutions. It also linearly increases in the gel phase but almost three times faster (the corresponding slopes are 4.9 nN/100 mM and 13.32 nN/100 mM, respectively). We also showed that in the presence of divalent ions (Ca2+ and Mg2+) the bilayer mechanical hardness was unproportionally increased, and that was accompanied with the decrease of Na+ ion and increase of Cl− ion bound lipids. The underlying process is a cooperative and competitive ion binding in both the gel and the liquid crystalline phase. Bilayer hardness thus turned out to be very sensitive to ionic strength as well as to ionic composition of the surrounding medium. In particular, the indicated correlation helped us to emphasize the colligative properties of SW as a naturally occurring complex ion mixture.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Kemija, Biologija



POVEZANOST RADA


Projekt / tema
098-0982904-2927 - Makrociklički ligandi, strukturne promjene otopina i molekularne spektroskopije (Goran Baranović, )
098-0982934-2744 - Površinske sile na atomskoj skali u istraživanju mora i nanotehnologiji (Vesna Svetličić, )

Ustanove
Institut "Ruđer Bošković", Zagreb

Citiraj ovu publikaciju

Šegota, Suzana; Vojta, Danijela; Pletikapić, Galja; Baranović, Goran
Ionic strength and composition govern the elasticity of biological membranes. A study of model DMPC bilayers by force- and transmission IR spectroscopy // Chemistry and physics of lipids, 186 (2015), 17-29 doi:10.1016/j.chemphyslip.2014.11.001 (međunarodna recenzija, članak, znanstveni)
Šegota, S., Vojta, D., Pletikapić, G. & Baranović, G. (2015) Ionic strength and composition govern the elasticity of biological membranes. A study of model DMPC bilayers by force- and transmission IR spectroscopy. Chemistry and physics of lipids, 186, 17-29 doi:10.1016/j.chemphyslip.2014.11.001.
@article{article, year = {2015}, pages = {17-29}, DOI = {10.1016/j.chemphyslip.2014.11.001}, keywords = {AFM, Force spectroscopy, Ion binding, Ion mixture, IR spectroscopy, Lipid bilayers, Nanomechanics, Seawater}, journal = {Chemistry and physics of lipids}, doi = {10.1016/j.chemphyslip.2014.11.001}, volume = {186}, issn = {0009-3084}, title = {Ionic strength and composition govern the elasticity of biological membranes. A study of model DMPC bilayers by force- and transmission IR spectroscopy}, keyword = {AFM, Force spectroscopy, Ion binding, Ion mixture, IR spectroscopy, Lipid bilayers, Nanomechanics, Seawater} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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