Deep scanning for GDSL motifs across ecologically diverse Actinobacteria (CROSBI ID 619315)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Bielen, Ana ; Bruvo-Mađarić, Branka ; Vujaklija, Ivan ; Paradžik, Tina ; Biđin, Siniša ; Pezer Sakač, Željka ; Goldstein, Pavle ; Vujaklija, Dušica
engleski
Deep scanning for GDSL motifs across ecologically diverse Actinobacteria
Enzymes of GDSL family exhibit high potential for pharmaceutical and biotechnological applications, due to their broad substrate specificities and diverse catalytic activities like lipase, phospholipase, esterase and thioesterase. They are characterized by five distinct protein motifs (or Blocks), among which Block I, III and V exhibit higher conservation and carry important catalytic residues. The number of proteins assigned to the GDSL family has increased rapidly over the last few years and continues to expand. Preliminary analysis of Uniprot database showed that Actinobacteria could be a rich source of GDSL hydrolases. Due to low overall sequence similarity within members of the GDSL family, we used motif scanning as a tool for identification of actinobacterial GDSL sequences. We scanned 77 actinobacterial proteomes and found 484 GDSL enzymes in total (up to 26 per proteome). CLANS clustering and phylogenetic analysis provided new insights into evolution of GDSL family in Actinobacteria. Newly identified protein sequences formed eight well defined groups, each possessing undescribed variations in GDSL motifs that possibly reflect novel enzyme properties of biotechnological interest. Further, our results pointed out that horizontal gene transfer had a significant impact in evolution of actinobacterial GDSL genes. These bacteria exhibit a variety of lifestyles and acquiring multiple GDSL genes by horizontal transfer could significantly help them in their saprophytic lifestyle in soil and in adaptation to novel ecological niches.
GDSL lipolytic family; motif scanning; Actinobacteria
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Podaci o prilogu
2014.
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objavljeno
Podaci o matičnoj publikaciji
XVII. International Symposium on the Biology of Actinomycetes (ISBA'17) Book of Abstracts
Podaci o skupu
XVII. International Symposium on the Biology of Actinomycetes (ISBA'17)
poster
01.01.2014-01.01.2014
Turska