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izvor podataka: crosbi !

Deep scanning for GDSL motifs across ecologically diverse Actinobacteria (CROSBI ID 619315)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Bielen, Ana ; Bruvo-Mađarić, Branka ; Vujaklija, Ivan ; Paradžik, Tina ; Biđin, Siniša ; Pezer Sakač, Željka ; Goldstein, Pavle ; Vujaklija, Dušica Deep scanning for GDSL motifs across ecologically diverse Actinobacteria // XVII. International Symposium on the Biology of Actinomycetes (ISBA'17) Book of Abstracts. 2014

Podaci o odgovornosti

Bielen, Ana ; Bruvo-Mađarić, Branka ; Vujaklija, Ivan ; Paradžik, Tina ; Biđin, Siniša ; Pezer Sakač, Željka ; Goldstein, Pavle ; Vujaklija, Dušica

engleski

Deep scanning for GDSL motifs across ecologically diverse Actinobacteria

Enzymes of GDSL family exhibit high potential for pharmaceutical and biotechnological applications, due to their broad substrate specificities and diverse catalytic activities like lipase, phospholipase, esterase and thioesterase. They are characterized by five distinct protein motifs (or Blocks), among which Block I, III and V exhibit higher conservation and carry important catalytic residues. The number of proteins assigned to the GDSL family has increased rapidly over the last few years and continues to expand. Preliminary analysis of Uniprot database showed that Actinobacteria could be a rich source of GDSL hydrolases. Due to low overall sequence similarity within members of the GDSL family, we used motif scanning as a tool for identification of actinobacterial GDSL sequences. We scanned 77 actinobacterial proteomes and found 484 GDSL enzymes in total (up to 26 per proteome). CLANS clustering and phylogenetic analysis provided new insights into evolution of GDSL family in Actinobacteria. Newly identified protein sequences formed eight well defined groups, each possessing undescribed variations in GDSL motifs that possibly reflect novel enzyme properties of biotechnological interest. Further, our results pointed out that horizontal gene transfer had a significant impact in evolution of actinobacterial GDSL genes. These bacteria exhibit a variety of lifestyles and acquiring multiple GDSL genes by horizontal transfer could significantly help them in their saprophytic lifestyle in soil and in adaptation to novel ecological niches.

GDSL lipolytic family; motif scanning; Actinobacteria

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Podaci o prilogu

2014.

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objavljeno

Podaci o matičnoj publikaciji

XVII. International Symposium on the Biology of Actinomycetes (ISBA'17) Book of Abstracts

Podaci o skupu

XVII. International Symposium on the Biology of Actinomycetes (ISBA'17)

poster

01.01.2014-01.01.2014

Turska

Povezanost rada

Biologija