engleski

COMPOSITION OF SOLUBLE SACCHARIDES IN SOYBEAN GRAIN ACCORDING TO GROWING CONDITIONS

Soybeans have long been recognized not only as a valuable source of edible oil but also as an excellent source of protein for feeding both animals and humans. The feed value of soybean, however, is limited by the of oligosaccharides because of flatulence based on their indigestibility. As oligosaccharides act as substrate for intestinal bacteria, they are also considered as prebiotics. The objective of this study was to evaluate the composition of soluble saccharides (monosaccharides, disaccharides and oligosaccharides) of four soybean genotypes created at the Agricultural institute Osijek as influenced by irrigation and nitrogen fertilization. Soybean samples were ground and soluble saccharides were extracted with water and separated by high performance liquid chromatography. Saccharides were identified by their retention time and quantified by peak area using external standard procedure. Analysis of variance showed significant effect (p≥ 0.05) of genotype and irrigation on concentration of stachyose, raffinose, sucrose, fructose and total oligosaccharides. The interaction between genotype, irrigation and nitrogen fertilization also revealed a significant effect (p≥ 0.05) on these grain compositions. Among the sugars studied, the highest content in soybean was sucrose, which was 46.64% of total saccharides (mean of all treatments). Oligosaccharides are represented with 45.48% of the total saccharides, of which stachyose occupy 32.03% and raffinose 13.45%. From monosaccharides in soybean grain, low concentration of glucose, galactose and fructose was also found.

soybean; irrigation; nitrogen fertilization; oligosaccharides

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