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Pregled bibliografske jedinice broj: 737455

Influence of Differences in Experimental Structure Annotations on Accuracy of Structure Prediction of Membrane Proteins


Batista, Jadranko; Lučić, Bono
Influence of Differences in Experimental Structure Annotations on Accuracy of Structure Prediction of Membrane Proteins // Proceedings of the First Adriatic Symposium on Biophysical Approaches in Biomedical Studies / Raguž, Marija ; Kalyanaramam, Balaraman ; Sarna, Tadeusz ; Ilić, Nada ; Nejašmić, Danijel ; Thelaner, Jane (ur.).
Split: Mediterranean Institute for Life Sciences, 2014. str. 60-60 (poster, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 737455 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Influence of Differences in Experimental Structure Annotations on Accuracy of Structure Prediction of Membrane Proteins

Autori
Batista, Jadranko ; Lučić, Bono

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Proceedings of the First Adriatic Symposium on Biophysical Approaches in Biomedical Studies / Raguž, Marija ; Kalyanaramam, Balaraman ; Sarna, Tadeusz ; Ilić, Nada ; Nejašmić, Danijel ; Thelaner, Jane - Split : Mediterranean Institute for Life Sciences, 2014, 60-60

ISBN
978-953-55188-2-2

Skup
First Adriatic Symposium on Bipphysical Approaches in Biomedical Studies

Mjesto i datum
Split, Hrvatska, 24-29.08.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
selection ; representative set ; membrane proteins ; secondary structure ; validation ; prediction method ; redundancy ; transmembrane segments

Sažetak
Selection of representative set of proteins to be used for training and validation of bioinformatics method is a key starting step in development of novel reliable methods for prediction of secondary structure of membrane proteins. In the past, and even nowadays, the number of experimentally solved and non-redundant structures of membrane proteins is relatively low, and definitely much lower than the number of unique soluble proteins. Additionally, location of transmembrane segments (either those in alpha or in beta secondary structure) for protein of known structure is not straightforward task. Consequently, the same proteins in different protein databases can have (more or less) different annotations of secondary structure. In this study, starting from information of PDB (Protein Data Bank, http://www.rcsb.org), UNIPROT (http://www.uniprot.org/), and OPM (Orientations of Proteins in Membranes, http://opm.phar.umich.edu) databases, and using ClustalOmega algorithm (http://www.ebi.ac.uk/Tools/msa/clustalo) we created novel representative set of membrane proteins of alpha class at the level of redundancy (in primary structures) below 30%. For this set of membrane proteins we extracted UNIPROT and OPM secondary structure and topology annotations, and all representative sequences were submitted to several representative prediction methods at the http://single.topcons.net/. Accuracies of prediction methods were evaluated in terms of two initial annotations of secondary structure. It was found that significant differences exist beween two experimental structure annotations (i.e. UNIPROT and OPM) and between prediction methods for several membrane proteins.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Kemija, Biologija



POVEZANOST RADA


Projekti:
MZO-Croatia - basic grant
Zaklada HAZU
098-1770495-2919 - Razvoj metoda za modeliranje svojstava bioaktivnih molekula i proteina (Lučić, Bono, MZOS ) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Bono Lučić (autor)

Citiraj ovu publikaciju

Batista, Jadranko; Lučić, Bono
Influence of Differences in Experimental Structure Annotations on Accuracy of Structure Prediction of Membrane Proteins // Proceedings of the First Adriatic Symposium on Biophysical Approaches in Biomedical Studies / Raguž, Marija ; Kalyanaramam, Balaraman ; Sarna, Tadeusz ; Ilić, Nada ; Nejašmić, Danijel ; Thelaner, Jane (ur.).
Split: Mediterranean Institute for Life Sciences, 2014. str. 60-60 (poster, međunarodna recenzija, sažetak, znanstveni)
Batista, J. & Lučić, B. (2014) Influence of Differences in Experimental Structure Annotations on Accuracy of Structure Prediction of Membrane Proteins. U: Raguž, M., Kalyanaramam, B., Sarna, T., Ilić, N., Nejašmić, D. & Thelaner, J. (ur.)Proceedings of the First Adriatic Symposium on Biophysical Approaches in Biomedical Studies.
@article{article, year = {2014}, pages = {60-60}, keywords = {selection, representative set, membrane proteins, secondary structure, validation, prediction method, redundancy, transmembrane segments}, isbn = {978-953-55188-2-2}, title = {Influence of Differences in Experimental Structure Annotations on Accuracy of Structure Prediction of Membrane Proteins}, keyword = {selection, representative set, membrane proteins, secondary structure, validation, prediction method, redundancy, transmembrane segments}, publisher = {Mediterranean Institute for Life Sciences}, publisherplace = {Split, Hrvatska} }
@article{article, year = {2014}, pages = {60-60}, keywords = {selection, representative set, membrane proteins, secondary structure, validation, prediction method, redundancy, transmembrane segments}, isbn = {978-953-55188-2-2}, title = {Influence of Differences in Experimental Structure Annotations on Accuracy of Structure Prediction of Membrane Proteins}, keyword = {selection, representative set, membrane proteins, secondary structure, validation, prediction method, redundancy, transmembrane segments}, publisher = {Mediterranean Institute for Life Sciences}, publisherplace = {Split, Hrvatska} }




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