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Determination of Free and Bound Monoterpenes Content in Aromatic Plants With Simple Distillation-spectrophotometric Method

In this paper the total content of free volatile monoterpenes (FVT) and glycosidically bound monoterpenes (GBT) present in six aromatic plants belonging to Lamiaceae family was determined by using simple and rapid distillation- spectrophotometric method. The investigated wild- growing plants, namely sage, lavender, rosemary, savory and oregano, were collected around Split during spring 2014., while commercial sample of basil was used. The method was originally developed for determination of free and glycosidically bound terpenes in grapes and adapted to analyze these compounds in aromatic plants. The plant materials were hydrodistilled under neutral and acidic conditions to collect two fractions, FVT and GBT. The colorimetric determination of monoterpenes in distillates was performed by adding vanillin-sulphuric acid reagent in order to obtain colour. The intensity of the colour was proportional to the content of monoterpenes. Absorbance was measured at 608 nm. The contents of monoterpenes in the distillates were calculated from the standard curve prepared with linalool standard solutions. According to the obtained results, all investigated plants contained very similar contents of FVT and GBT, except oregano with the lowest contents of these monoterpenes. Only slightly higher contents of FVT and GBT was recorded in basil.

free volatile monoterpenes ; glycosidically bound monoterpenes ; aromatic plants ; distillation-spectrophotometric method

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